PMID- 24028025 OWN - NLM STAT- MEDLINE DCOM- 20140925 LR - 20161018 IS - 0250-3301 (Print) IS - 0250-3301 (Linking) VI - 34 IP - 7 DP - 2013 Jul TI - [Rapid enrichment and cultivation of denitrifying phosphate-removal bacteria and its identification by fluorescence in situ hybridization technology]. PG - 2869-75 AB - The present work focused on a rapid enrichment and cultivation of denitrifying phosphate-removal bacteria (DPB) in a membrane bio-reactor(MBR) by using A2/O anaerobic sludge from a wastewater treatment plant as seed, as well as providing an identification method. In the experiments, sodium acetate was used as the carbon source and a certain amount of nitrate was added to the MBR in the anoxic stage. Results showed that, with the efficient trap of the hollow-fiber membrane module, the proportion of DPB in all the phosphate-accumulating organisms (PAOs) increased from 24% to 93% within 35 days after two-stage's cultivation including anaerobic/aerobic and anaerobic/anoxic, during which the removal efficiency of nitrogen and phosphorus reached more than 90%. The activated sludge was identified by combining a regular method and the fluorescence in situ hybridization (FISH) technique, which demonstrated that Pseudomonas sp. and Rhodocyclus sp. were the dominant bacteria in the used bioreactor. FAU - Liu, Li AU - Liu L AD - School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China. liuli19880128@163.com FAU - Tang, Bing AU - Tang B FAU - Huang, Shao-Song AU - Huang SS FAU - Fu, Feng-Lian AU - Fu FL FAU - Zhang, Qi-Qin AU - Zhang QQ FAU - Li, Jian-Bin AU - Li JB FAU - Luo, Jian-Zhong AU - Luo JZ LA - chi PT - English Abstract PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - China TA - Huan Jing Ke Xue JT - Huan jing ke xue= Huanjing kexue JID - 8405344 RN - 0 (Sewage) RN - 27YLU75U4W (Phosphorus) RN - N762921K75 (Nitrogen) SB - IM MH - Aerobiosis MH - Anaerobiosis MH - Bacteria/growth & development/isolation & purification/*metabolism MH - Bioreactors/microbiology MH - *Denitrification MH - In Situ Hybridization, Fluorescence MH - Nitrogen/*isolation & purification/metabolism MH - Phosphorus/*isolation & purification/metabolism MH - Pseudomonas/growth & development/isolation & purification/metabolism MH - Rhodocyclaceae/growth & development/isolation & purification/metabolism MH - Sewage/*microbiology MH - Waste Disposal Facilities MH - Waste Disposal, Fluid/methods EDAT- 2013/09/14 06:00 MHDA- 2014/09/26 06:00 CRDT- 2013/09/14 06:00 PHST- 2013/09/14 06:00 [entrez] PHST- 2013/09/14 06:00 [pubmed] PHST- 2014/09/26 06:00 [medline] PST - ppublish SO - Huan Jing Ke Xue. 2013 Jul;34(7):2869-75.