PMID- 2402872
OWN - NLM
STAT- MEDLINE
DCOM- 19901026
LR  - 20190828
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 23
IP  - 1-4
DP  - 1990 Jun
TI  - Molecular cloning of the mRNA coding for the G protein of the viral haemorrhagic 
      septicaemia (VHS) of salmonids.
PG  - 221-6
AB  - Viral haemorrhagic septicaemia virus (VHSV), a rhabdovirus, is a major threat for 
      continental European trout fish farming. The development of a recombinant subunit 
      vaccine could solve that problem. The neutralizing epitopes are located on the 
      glycoprotein or G protein, the surface antigen. The G protein has a molecular 
      weight of 65 kDa, reduced to 55 kDa by deglycosylation. cDNA was synthetized from 
      mRNA of VHS virus infected cells, and cloned in E. coli. The viral cDNA was 
      recognized by positive hybridization with a labelled probe made from infected 
      cell RNA, and negative hybridization with labelled cDNA made from cellular RNA. 
      The Northern blot hybridization with different clones on VHS infected cell RNA 
      revealed two VHS mRNA whose lengths, 2.0 and 1.5 kb, were compatible with the 
      mRNA length for G and N proteins respectively. This mRNA must contain about 400 
      bp of untranslated sequence.
FAU - Thiry, M
AU  - Thiry M
AD  - EUROGENTEC SA, Liege, Belgium.
FAU - Lecocq-Xhonneux, F
AU  - Lecocq-Xhonneux F
FAU - Dheur, I
AU  - Dheur I
FAU - Renard, A
AU  - Renard A
FAU - de Kinkelin, P
AU  - de Kinkelin P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (DNA, Viral)
RN  - 0 (G protein, viral hemorrhagic septicemia virus)
RN  - 0 (Glycoproteins)
RN  - 0 (RNA Probes)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Envelope Proteins)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cloning, Molecular
MH  - DNA, Viral/genetics
MH  - Fish Diseases/microbiology
MH  - Glycoproteins/*genetics
MH  - Nucleic Acid Hybridization
MH  - Plasmids
MH  - RNA Probes
MH  - RNA, Messenger/genetics
MH  - RNA, Viral/*genetics
MH  - Rhabdoviridae/*genetics
MH  - Trout
MH  - Viral Envelope Proteins/*genetics
MH  - Virus Diseases/microbiology/veterinary
EDAT- 1990/06/01 00:00
MHDA- 1990/06/01 00:01
CRDT- 1990/06/01 00:00
PHST- 1990/06/01 00:00 [pubmed]
PHST- 1990/06/01 00:01 [medline]
PHST- 1990/06/01 00:00 [entrez]
AID - 0378-1135(90)90152-L [pii]
AID - 10.1016/0378-1135(90)90152-l [doi]
PST - ppublish
SO  - Vet Microbiol. 1990 Jun;23(1-4):221-6. doi: 10.1016/0378-1135(90)90152-l.