PMID- 24028897 OWN - NLM STAT- MEDLINE DCOM- 20140509 LR - 20130930 IS - 1873-2720 (Electronic) IS - 0944-2006 (Linking) VI - 116 IP - 5 DP - 2013 Oct TI - Genomic analyses of the Formosan harvest mouse (Micromys minutus) and comparisons to the brown Norway rat (Rattus norvegicus) and the house mouse (Mus musculus). PG - 307-15 LID - S0944-2006(13)00067-6 [pii] LID - 10.1016/j.zool.2013.07.001 [doi] AB - The harvest mouse, Micromys minutus (MMIN), has a very wide range of distribution (from the British Isles across the Euroasian continent to Japan and Taiwan). We studied an isolated population of MMIN in Taiwan, which is at the southeastern margin of the species' geographic distribution, and compared its genetic complement with those of the same species previously reported from other geographic locations and with two model rodent species, the house mouse (Mus musculus) and the brown Norway rat (Rattus norvegicus). The diploid number (2N) of MMIN was 68, consistent with that reported for other populations. However, variations were noted in the fundamental number (FN) and the shape and banding patterns of the individual chromosomes among populations. The FN of MMIN was estimated to be 72, including 2 bi-armed autosomes, 31 one-armed autosomes, and one pair of one-armed sex chromosomes. Here, we propose the first ideogram for MMIN. C-banding, Ag-NOR, and the locations of 18S rRNA gene sequences (MMIN chromosomes no. 10, 14, 19, 29, 31, 33, and X) mapped by fluorescence in situ hybridization (FISH) are also reported. Additionally, we compared the 18S rDNA sequences and performed cross-species X chromosome painting (FISH) for M. minutus, M. musculus, and R. norvegicus. The results indicate that both genetic elements are rather conserved across species. Thus, implications for the phylogenetic position of Micromys were limited. CI - Copyright (c) 2013 Elsevier GmbH. All rights reserved. FAU - Lin, Liang-Kong AU - Lin LK AD - Department of Life Sciences, Tunghai University, Taichung, Taiwan. FAU - Ma, Gwo-Chin AU - Ma GC FAU - Chen, Tze-Ho AU - Chen TH FAU - Lin, Wen-Hsiang AU - Lin WH FAU - Lee, Dong-Jay AU - Lee DJ FAU - Wen, Pao-Ying AU - Wen PY FAU - Wu, Sheng-Hai AU - Wu SH FAU - Chen, Ming AU - Chen M LA - eng PT - Comparative Study PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130828 PL - Germany TA - Zoology (Jena) JT - Zoology (Jena, Germany) JID - 9435608 RN - 0 (RNA, Ribosomal, 18S) SB - IM MH - Animals MH - Base Sequence MH - Chromosome Painting MH - *Chromosomes, Mammalian MH - Female MH - *Genome MH - Male MH - Mice MH - Molecular Sequence Data MH - Murinae/*genetics MH - RNA, Ribosomal, 18S/*genetics MH - Rats OTO - NOTNLM OT - 18S rRNA OT - Comparative genetics OT - FISH OT - Genomic analysis OT - Harvest mouse EDAT- 2013/09/14 06:00 MHDA- 2014/05/10 06:00 CRDT- 2013/09/14 06:00 PHST- 2012/10/17 00:00 [received] PHST- 2013/06/13 00:00 [revised] PHST- 2013/07/13 00:00 [accepted] PHST- 2013/09/14 06:00 [entrez] PHST- 2013/09/14 06:00 [pubmed] PHST- 2014/05/10 06:00 [medline] AID - S0944-2006(13)00067-6 [pii] AID - 10.1016/j.zool.2013.07.001 [doi] PST - ppublish SO - Zoology (Jena). 2013 Oct;116(5):307-15. doi: 10.1016/j.zool.2013.07.001. Epub 2013 Aug 28.