PMID- 24029475
OWN - NLM
STAT- MEDLINE
DCOM- 20140310
LR  - 20161125
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 93
IP  - 17
DP  - 2013 May 7
TI  - [Experimental autoimmune model of muscle-specific kinase specific myasthenia 
      gravis in rats].
PG  - 1292-6
AB  - OBJECTIVE: To establish the experimental autoimmune Lewis rat model of myasthenia 
      gravis (EAMG) specific for muscle-specific kinase (MuSK). METHODS: Lewis rats 
      were immunized subcutaneously with a single injection of 200 microl of MuSK (50/100 
      microg) emulsified in complete Freund's adjuvant (CFA). And MuSK was expressed 
      according to the murine gene of MuSK (AY360453). Control rats were immunized with 
      CFA alone. All rats were observed every other day in a blinded fashion for 
      fatigue characteristics. The clinical symptoms were graded between 0 and 3. And 
      the clinical scores were recorded. The serum levels of anti-MuSK IgG at Days 7 
      and 57 were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: The 
      clinical symptoms of myasthenia gravis (MG) appeared at Day 16 and peaked at Days 
      18-22. The serum levels of anti-MuSK IgG were markedly elevated in the 
      experimental groups compared with the control group. CONCLUSION: EAMG induced by 
      MuSK is successfully established in rats. And this animal model may help us 
      elucidate the pathogenesis of anti-MuSK myasthenia (AMM) and discover new drug 
      therapy in the future.
FAU - Chen, Yu
AU  - Chen Y
AD  - Department of Neurology, Beijing Tiantan Hospital, Capital Medical University, 
      Beijing 100050, China.
FAU - Wang, Hua-bing
AU  - Wang HB
FAU - Wang, Yong-jun
AU  - Wang YJ
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (Autoantibodies)
RN  - 0 (Receptors, Cholinergic)
RN  - EC 2.7.10.1 (MuSK protein, rat)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
SB  - IM
MH  - Animals
MH  - Autoantibodies/*immunology
MH  - *Disease Models, Animal
MH  - Female
MH  - *Myasthenia Gravis, Autoimmune, Experimental
MH  - Rats
MH  - Rats, Inbred Lew
MH  - Receptor Protein-Tyrosine Kinases/*immunology
MH  - Receptors, Cholinergic/*immunology
EDAT- 2013/09/14 06:00
MHDA- 2014/03/13 06:00
CRDT- 2013/09/14 06:00
PHST- 2013/09/14 06:00 [entrez]
PHST- 2013/09/14 06:00 [pubmed]
PHST- 2014/03/13 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2013 May 7;93(17):1292-6.