PMID- 24029776
OWN - NLM
STAT- MEDLINE
DCOM- 20140414
LR  - 20211021
IS  - 2210-7185 (Electronic)
IS  - 2210-7177 (Print)
IS  - 2210-7177 (Linking)
VI  - 36
IP  - 3-4
DP  - 2013
TI  - Urothelial differentiation of human umbilical cord-derived mesenchymal stromal 
      cells in vitro.
PG  - 63-9
LID - 10.3233/ACP-130081 [doi]
AB  - Human umbilical cord-derived mesenchymal stromal cells (hUCMSCs) are the most 
      primitive of those isolated from other post-natal tissue source. The hUCMSCs 
      possess the capability of differentiating along multi-lineage. This study aimed 
      to investigate whether hUCMSCs can differentiate into urothelium-like cells. The 
      hUCMSCs were isolated from fresh human umbilical cord postpartum and expanded at 
      least to passage 3 in vitro. Subsequently, they were cultured with conditioned 
      medium from urothelial cells (UC-CM) supplemented with 20 ng/ml exogenous 
      epidermal growth factor (EGF). Urothelial cell specific marker uroplakin II 
      (UPII) and cytokeratins were evaluated by reverse transcriptase-polymerase chain 
      reaction (RT-PCR) and immunofluorescence technology. During culture, hUCMSCs 
      started to express UPII and cytokeratins weakly at 7 days and were significantly 
      up-regulated at 2 weeks post-induction. Additionally, morphology of hUCMSCs 
      changed from spindle-shape to a polygonal epithelial-shape similar to that of 
      urothelial cells after 7 days. The study results indicated that hUCMSCs can 
      differentiate into urothelium-like cells in a defined micro-environment in vitro 
      constituted by UC-CM and exogenous EGF.
FAU - Wu, Shuai
AU  - Wu S
AD  - School of Medicine, Shanghai First People's Hospital, Shanghai Jiao Tong 
      University, Shanghai, China Department of Urology, Shanghai First People's 
      Hospital, Shanghai Jiao Tong University, Shanghai, China Department of Urology, 
      Qingdao Municipal Hospital, Qingdao, China.
FAU - Cheng, Zhongliang
AU  - Cheng Z
FAU - Liu, Guohua
AU  - Liu G
FAU - Zhao, Xinfeng
AU  - Zhao X
FAU - Zhong, Liang
AU  - Zhong L
FAU - Zhu, Yingjian
AU  - Zhu Y
FAU - Zhu, Jiang
AU  - Zhu J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Cell Pathol (Amst)
JT  - Analytical cellular pathology (Amsterdam)
JID - 101541993
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (RNA, Messenger)
RN  - 0 (UPK2 protein, human)
RN  - 0 (Uroplakin II)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 68238-35-7 (Keratins)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Lineage
MH  - Cells, Cultured
MH  - Cellular Microenvironment
MH  - Culture Media, Conditioned/pharmacology
MH  - Epidermal Growth Factor/pharmacology
MH  - Gene Expression Regulation, Developmental
MH  - Humans
MH  - In Vitro Techniques
MH  - Infant, Newborn
MH  - Keratins/biosynthesis/genetics
MH  - Mesenchymal Stem Cells/*cytology/drug effects/metabolism
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Umbilical Cord/cytology
MH  - Uroplakin II/biosynthesis/genetics
MH  - Urothelium/*cytology/metabolism
PMC - PMC4605704
OTO - NOTNLM
OT  - Cell differentiation
OT  - mesenchymal stromal cells
OT  - umbilical cord
OT  - urothelium
EDAT- 2013/09/14 06:00
MHDA- 2014/04/15 06:00
PMCR- 2013/09/12
CRDT- 2013/09/14 06:00
PHST- 2013/09/14 06:00 [entrez]
PHST- 2013/09/14 06:00 [pubmed]
PHST- 2014/04/15 06:00 [medline]
PHST- 2013/09/12 00:00 [pmc-release]
AID - C7G46134733362XW [pii]
AID - 274640 [pii]
AID - 10.3233/ACP-130081 [doi]
PST - ppublish
SO  - Anal Cell Pathol (Amst). 2013;36(3-4):63-9. doi: 10.3233/ACP-130081.