PMID- 24032570
OWN - NLM
STAT- MEDLINE
DCOM- 20140513
LR  - 20130916
IS  - 1651-2251 (Electronic)
IS  - 0001-6489 (Linking)
VI  - 133
IP  - 10
DP  - 2013 Oct
TI  - Multiplex analyses of cytokine and chemokine release from the cultured fibroblast 
      of nasal polyps: the effect of IL-17A.
PG  - 1065-72
LID - 10.3109/00016489.2013.796091 [doi]
AB  - CONCLUSION: Our results demonstrate for the first time a potentially enhanced 
      basal secretion of monocyte chemoattractant protein-1 (MCP-1) and interleukin 
      (IL)-17A-stimulated secretion of IL-6 from nasal polyp fibroblasts, enhanced 
      basal secretion of IL-6 from eosinophilic nasal polyp fibroblasts, and a 
      remarkable up-regulation of IL-9 and granulocyte colony-stimulating factor 
      (G-CSF) from nasal fibroblasts by IL-17A stimulation. OBJECTIVES: The fibroblast, 
      one of the main cell types making up nasal polyps, is thought to be a target cell 
      of various cytokines. METHODS: Subcultured fibroblasts were established from 
      human polyp biopsy tissues. Simultaneous quantification of 27 kinds of cytokines 
      and chemokines in culture supernatants in unstimulated and IL-17A-stimulated 
      conditions was performed with a human multiplex cytokine assay system. RESULTS: 
      The IL-17A receptor was expressed at similar levels in all three groups. In the 
      eosinophilic group, basal secretion levels of IL-6 were significantly higher than 
      those in the control and non-eosinophilic groups. Basal secretion of MCP-1 in 
      both the non-eosinophilic and eosinophilic groups was also higher than that of 
      the control group. Both IL-9 and G-CSF secretion were remarkably enhanced by 
      IL-17A stimulation in all three groups. The receptor-mediated response by IL-17A 
      significantly up-regulated IL-6 release alone in the non-eosinophilic and 
      eosinophilic groups as compared with the control group.
FAU - Homma, Hirotomo
AU  - Homma H
AD  - Department of Otorhinolaryngology, Juntendo University Faculty of Medicine , 
      Tokyo , Japan.
FAU - Kamiya, Kazusaku
AU  - Kamiya K
FAU - Kusunoki, Takeshi
AU  - Kusunoki T
FAU - Ikeda, Katsuhisa
AU  - Ikeda K
LA  - eng
PT  - Journal Article
PL  - England
TA  - Acta Otolaryngol
JT  - Acta oto-laryngologica
JID - 0370354
RN  - 0 (Chemokines)
RN  - 0 (Cytokines)
RN  - 0 (Interleukin-17)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Cells, Cultured
MH  - Chemokines/*biosynthesis
MH  - Cytokines/biosynthesis
MH  - Female
MH  - Fibroblasts/metabolism/pathology
MH  - *Gene Expression Regulation
MH  - Humans
MH  - Immunoassay
MH  - Interleukin-17/biosynthesis/*genetics/pharmacology
MH  - Male
MH  - Middle Aged
MH  - Nasal Polyps/genetics/*metabolism/pathology
MH  - RNA, Messenger/*genetics
MH  - Real-Time Polymerase Chain Reaction
MH  - Young Adult
EDAT- 2013/09/17 06:00
MHDA- 2014/05/14 06:00
CRDT- 2013/09/17 06:00
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2014/05/14 06:00 [medline]
AID - 10.3109/00016489.2013.796091 [doi]
PST - ppublish
SO  - Acta Otolaryngol. 2013 Oct;133(10):1065-72. doi: 10.3109/00016489.2013.796091.