PMID- 24032685
OWN - NLM
STAT- MEDLINE
DCOM- 20140403
LR  - 20181202
IS  - 1208-6002 (Electronic)
IS  - 0829-8211 (Linking)
VI  - 91
IP  - 5
DP  - 2013 Oct
TI  - BDNF protects human vascular endothelial cells from TNFalpha-induced apoptosis.
PG  - 341-9
LID - 10.1139/bcb-2013-0005 [doi]
AB  - Brain-derived neurotrophic factor (BDNF) enhances periodontal tissue 
      regeneration. Tissue regeneration is characterized by inflammation that directs 
      the quality of tissue repair. In this study, we investigated the anti-apoptotic 
      effect of BDNF against the toxicity of tumor necrosis factor alpha (TNFalpha), which is 
      known for its pro-apoptotic action in human microvascular endothelial cells 
      (HMVECs). We demonstrate that BDNF attenuates TNFalpha-increased Annexin V-positive 
      cells, lactic dehydrogenase (LDH) release, and intercellular adhesion molecule 1 
      (ICAM-1) mRNA and cleaved caspase-3 expression. In addition, biochemical analyses 
      indicate that TNFalpha increases phosphatase and tensin homolog (PTEN) expression; 
      however, it decreases phosphorylated PTEN. BDNF did not affect PTEN expression, 
      but it did increase the phosphorylation of PTEN. BDNF-induced Akt phosphorylation 
      was inhibited by TNFalpha. Terminal deoxynucleotidyl transferase (TdT) dUTP nick end 
      labeling (TUNEL) assay showed that the PTEN inhibitor bpV(pic) rescues HMVECs 
      from TNFalpha-induced apoptosis. In conclusion, BDNF protects HMVECs from toxicity of 
      TNFalpha through the regulation of the PTEN/Akt pathway.
FAU - Takeda, Katsuhiro
AU  - Takeda K
AD  - a Division of Frontier Medical Science, Hiroshima University Graduate School of 
      Biomedical Sciences, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8553, Japan.
FAU - Kermani, Pouneh
AU  - Kermani P
FAU - Anastasia, Agustin
AU  - Anastasia A
FAU - Obinata, Yusuke
AU  - Obinata Y
FAU - Hempstead, Barbara L
AU  - Hempstead BL
FAU - Kurihara, Hidemi
AU  - Kurihara H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130610
PL  - Canada
TA  - Biochem Cell Biol
JT  - Biochemistry and cell biology = Biochimie et biologie cellulaire
JID - 8606068
RN  - 0 (Annexin A5)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Organometallic Compounds)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (oxodiperoxo(pyridine-2-carboxylate)vanadate(V))
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Annexin A5/metabolism
MH  - Apoptosis/*drug effects
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Caspase 3/biosynthesis/metabolism
MH  - Cell Line
MH  - *Cytoprotection
MH  - Endothelial Cells/*drug effects
MH  - Humans
MH  - Inflammation/metabolism
MH  - Intercellular Adhesion Molecule-1/genetics
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Organometallic Compounds/pharmacology
MH  - PTEN Phosphohydrolase/antagonists & inhibitors/biosynthesis/*metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/*metabolism
MH  - RNA, Messenger/biosynthesis
MH  - Regeneration/physiology
MH  - Signal Transduction
MH  - Tumor Necrosis Factor-alpha/*antagonists & inhibitors
EDAT- 2013/09/17 06:00
MHDA- 2014/04/04 06:00
CRDT- 2013/09/17 06:00
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2014/04/04 06:00 [medline]
AID - 10.1139/bcb-2013-0005 [doi]
PST - ppublish
SO  - Biochem Cell Biol. 2013 Oct;91(5):341-9. doi: 10.1139/bcb-2013-0005. Epub 2013 
      Jun 10.