PMID- 24033776
OWN - NLM
STAT- MEDLINE
DCOM- 20140603
LR  - 20171116
IS  - 1574-6968 (Electronic)
IS  - 0378-1097 (Linking)
VI  - 348
IP  - 2
DP  - 2013 Nov
TI  - Identification of active oxalotrophic bacteria by Bromodeoxyuridine DNA labeling 
      in a microcosm soil experiments.
PG  - 103-11
LID - 10.1111/1574-6968.12244 [doi]
AB  - The oxalate-carbonate pathway (OCP) leads to a potential carbon sink in 
      terrestrial environments. This process is linked to the activity of oxalotrophic 
      bacteria. Although isolation and molecular characterizations are used to study 
      oxalotrophic bacteria, these approaches do not give information on the active 
      oxalotrophs present in soil undergoing the OCP. The aim of this study was to 
      assess the diversity of active oxalotrophic bacteria in soil microcosms using the 
      Bromodeoxyuridine (BrdU) DNA labeling technique. Soil was collected near an 
      oxalogenic tree (Milicia excelsa). Different concentrations of calcium oxalate 
      (0.5%, 1%, and 4% w/w) were added to the soil microcosms and compared with an 
      untreated control. After 12 days of incubation, a maximal pH of 7.7 was measured 
      for microcosms with oxalate (initial pH 6.4). At this time point, a DGGE profile 
      of the frc gene was performed from BrdU-labeled soil DNA and unlabeled soil DNA. 
      Actinobacteria (Streptomyces- and Kribbella-like sequences), Gammaproteobacteria 
      and Betaproteobacteria were found as the main active oxalotrophic bacterial 
      groups. This study highlights the relevance of Actinobacteria as members of the 
      active bacterial community and the identification of novel uncultured 
      oxalotrophic groups (i.e. Kribbella) active in soils.
CI  - (c) 2013 Federation of European Microbiological Societies. Published by John Wiley 
      & Sons Ltd. All rights reserved.
FAU - Bravo, Daniel
AU  - Bravo D
AD  - Laboratory of Microbiology, Institute of Biology, University of Neuchatel, 
      Neuchatel, Switzerland.
FAU - Martin, Gaetan
AU  - Martin G
FAU - David, Maude M
AU  - David MM
FAU - Cailleau, Guillaume
AU  - Cailleau G
FAU - Verrecchia, Eric
AU  - Verrecchia E
FAU - Junier, Pilar
AU  - Junier P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130923
PL  - England
TA  - FEMS Microbiol Lett
JT  - FEMS microbiology letters
JID - 7705721
RN  - 0 (Carbonates)
RN  - 0 (DNA, Bacterial)
RN  - 0 (Soil)
RN  - 2612HC57YE (Calcium Oxalate)
RN  - G34N38R2N1 (Bromodeoxyuridine)
SB  - IM
MH  - Bacteria/*classification/*metabolism
MH  - Bromodeoxyuridine/analysis/chemistry/*metabolism
MH  - Calcium Oxalate/chemistry/*metabolism
MH  - Carbonates/chemistry/metabolism
MH  - DNA, Bacterial/chemistry/*metabolism
MH  - Hydrogen-Ion Concentration
MH  - Metabolic Networks and Pathways
MH  - Microbiota
MH  - Soil/chemistry
MH  - *Soil Microbiology
OTO - NOTNLM
OT  - BrdU
OT  - DGGE
OT  - Iroko
OT  - Milicia excelsa
OT  - oxalate-carbonate pathway
EDAT- 2013/09/17 06:00
MHDA- 2014/06/04 06:00
CRDT- 2013/09/17 06:00
PHST- 2013/06/10 00:00 [received]
PHST- 2013/08/19 00:00 [revised]
PHST- 2013/08/26 00:00 [accepted]
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2014/06/04 06:00 [medline]
AID - 10.1111/1574-6968.12244 [doi]
PST - ppublish
SO  - FEMS Microbiol Lett. 2013 Nov;348(2):103-11. doi: 10.1111/1574-6968.12244. Epub 
      2013 Sep 23.