PMID- 24036229
OWN - NLM
STAT- MEDLINE
DCOM- 20140707
LR  - 20211021
IS  - 1872-7492 (Electronic)
IS  - 0168-1702 (Print)
IS  - 0168-1702 (Linking)
VI  - 178
IP  - 2
DP  - 2013 Dec 26
TI  - Concurrent porcine circovirus type 2a (PCV2a) or PCV2b infection increases the 
      rate of amino acid mutations of porcine reproductive and respiratory syndrome 
      virus (PRRSV) during serial passages in pigs.
PG  - 445-51
LID - S0168-1702(13)00299-2 [pii]
LID - 10.1016/j.virusres.2013.09.007 [doi]
AB  - Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of 
      genetic and antigenic variability. The purpose of this study was to determine if 
      porcine circovirus type 2 (PCV2) infection increases genetic variability of PRRSV 
      during serial passages in pigs and to determine if there is a difference in the 
      PRRSV mutation rate between pigs concurrently infected with PCV2a or PCV2b. After 
      8 consecutive passages of PRRSV alone (group 1), PRRSV with PCV2a (group 2), or 
      PCV2b (group 3) in pigs, the sequences of PRRSV structural genes for open reading 
      frame (ORF) 5, ORF6, ORF7 and the partial non-structural protein gene (Nsp) 2 
      were determined. The total number of identified amino acid mutations in ORF5, 
      ORF6, ORF7 and Nsp2 sequences was 30 for PRRSV infection only, 63 for PRRSV/PCV2a 
      concurrent infection, and 77 for PRRSV/PCV2b concurrent infection when compared 
      with the original VR2385 virus used to infect the passage 1 pigs. Compared to 
      what occurred in pigs infected with PRRSV only, the mutation rates in ORF5 and 
      ORF6 were significantly higher for concurrent PRRSV/PCV2b infected pigs. The 
      PRRSV/PCV2a pigs had a significantly higher mutation rate in ORF7. The results 
      from this study indicated that, besides ORF5 and Nsp2, the PRRSV structural genes 
      ORF6 and ORF7 were shown to mutate at various degrees when the PRRSV was passaged 
      over time in vivo. Furthermore, a significantly higher mutation rate of PRRSV was 
      observed when pigs were co-infected with PCV2 highlighting the importance of 
      concurrent infections on PRRSV evolution and control.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Yin, Shuang-Hui
AU  - Yin SH
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, Ames, IA, USA.
FAU - Xiao, Chao-Ting
AU  - Xiao CT
FAU - Gerber, Priscilla F
AU  - Gerber PF
FAU - Beach, Nathan M
AU  - Beach NM
FAU - Meng, Xiang-Jin
AU  - Meng XJ
FAU - Halbur, Patrick G
AU  - Halbur PG
FAU - Opriessnig, Tanja
AU  - Opriessnig T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130913
PL  - Netherlands
TA  - Virus Res
JT  - Virus research
JID - 8410979
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - *Amino Acid Substitution
MH  - Animals
MH  - Circovirus/*growth & development
MH  - Coinfection/*virology
MH  - Mutation Rate
MH  - *Mutation, Missense
MH  - Porcine respiratory and reproductive syndrome virus/*genetics/*growth & 
      development
MH  - RNA, Viral/genetics
MH  - Sequence Analysis, DNA
MH  - Serial Passage
MH  - Swine
MH  - Viral Proteins/*genetics
PMC - PMC7126594
OTO - NOTNLM
OT  - Evolution
OT  - Mutation rate
OT  - Pigs
OT  - Porcine circovirus type 2
OT  - Porcine reproductive and respiratory syndrome virus
EDAT- 2013/09/17 06:00
MHDA- 2014/07/08 06:00
PMCR- 2013/09/13
CRDT- 2013/09/17 06:00
PHST- 2013/06/28 00:00 [received]
PHST- 2013/09/04 00:00 [revised]
PHST- 2013/09/04 00:00 [accepted]
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2014/07/08 06:00 [medline]
PHST- 2013/09/13 00:00 [pmc-release]
AID - S0168-1702(13)00299-2 [pii]
AID - 10.1016/j.virusres.2013.09.007 [doi]
PST - ppublish
SO  - Virus Res. 2013 Dec 26;178(2):445-51. doi: 10.1016/j.virusres.2013.09.007. Epub 
      2013 Sep 13.