PMID- 24037517
OWN - NLM
STAT- MEDLINE
DCOM- 20140702
LR  - 20131125
IS  - 1559-0291 (Electronic)
IS  - 0273-2289 (Linking)
VI  - 171
IP  - 8
DP  - 2013 Dec
TI  - Functional magnetic mesoporous nanoparticles for efficient purification of 
      laccase from fermentation broth in magnetically stabilized fluidized bed.
PG  - 2165-75
LID - 10.1007/s12010-013-0503-9 [doi]
AB  - A magnetically stabilized fluidized bed (MSFB) with the Cu(2+)-chelated magnetic 
      mesoporous silica nanoparticles (MMSNPs-Cu(2+)) was established to purify laccase 
      directly from the fermentation broth of Trametes versicolor. The MMSNPs-Cu(2+) 
      particles in the MSFB maintained a stable bed expansion of two to threefold at a 
      flow rate of 120-180 cm/h. At the optimal magnetic field intensity of 120 Gs, 
      both the maximal Bodenstein number and the smallest axial dispersion coefficient 
      were achieved, which resulted in a stable fluidization stage. The dynamic binding 
      capacity of laccase in the MSFB decreased from 192.5 to144.3 mg/g when the flow 
      velocity through the bed increased from 44.2 to 69.8 cm/h. The MSFB with 
      MMSNPs-Cu(2+) achieved efficient laccase purification from the fermentation broth 
      with 62.4-fold purification of laccase and 108.9 % activity yield. These results 
      provided an excellent platform for the application of these magnetic mesoporous 
      nanoparticles integrated with the MSFB in developing novel protein purification 
      process.
FAU - Wang, Feng
AU  - Wang F
AD  - National Key Laboratory of Biochemical Engineering, Institute of Process 
      Engineering, Chinese Academy of Sciences, Beijing, 100190, People's Republic of 
      China.
FAU - Guo, Chen
AU  - Guo C
FAU - Liu, Chun-Zhao
AU  - Liu CZ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130914
PL  - United States
TA  - Appl Biochem Biotechnol
JT  - Applied biochemistry and biotechnology
JID - 8208561
RN  - 0 (Magnetite Nanoparticles)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 1.10.3.2 (Laccase)
SB  - IM
MH  - Adsorption
MH  - Fermentation
MH  - Laccase/chemistry/*isolation & purification
MH  - Magnetic Fields
MH  - Magnetite Nanoparticles/*chemistry
MH  - Silicon Dioxide/chemistry
MH  - Trametes/*enzymology
EDAT- 2013/09/17 06:00
MHDA- 2014/07/06 06:00
CRDT- 2013/09/17 06:00
PHST- 2013/06/19 00:00 [received]
PHST- 2013/08/31 00:00 [accepted]
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2014/07/06 06:00 [medline]
AID - 10.1007/s12010-013-0503-9 [doi]
PST - ppublish
SO  - Appl Biochem Biotechnol. 2013 Dec;171(8):2165-75. doi: 10.1007/s12010-013-0503-9. 
      Epub 2013 Sep 14.