PMID- 24037850
OWN - NLM
STAT- MEDLINE
DCOM- 20140326
LR  - 20130916
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1060
DP  - 2014
TI  - Idiotype-specific intravenous immunoglobulin (IVIG) for therapy of autoimmune 
      diseases.
PG  - 353-61
LID - 10.1007/978-1-62703-586-6_18 [doi]
AB  - Intravenous immunoglobulin (IVIG) is used successfully for therapy of 
      inflammatory and autoimmune diseases, especially in cases of conventional therapy 
      resistance. Within the broad spectrum of immunomodulatory activities of IVIG in 
      vitro and in vivo, the anti-idiotypic activity, neutralizing the autoimmune 
      disease related idiotypes, is one of the main mechanism. We and others have 
      proven that from the IVIG composition, diverse fractions of autoimmune disease 
      specific IVIG can be affinity purified (sIVIG). This sIVIG was shown to be more 
      efficient than the whole compound of IVIG in experimental animal models of 
      autoimmune diseases.The affinity purification of disease sIVIG encompasses three 
      stages. The first stage is to construct an autoantigen column for affinity 
      purification of the autoantibodies. In the second stage the purified 
      autoantibodies are used to construct a new column composed of the autoantibodies. 
      The later is utilized for affinity purification of anti-autoantibodies (anti- 
      idiotypes) IVIG defined as autoimmune disease specific IVIG- sIVIG.
FAU - Blank, Miri
AU  - Blank M
AD  - Zabludowicz Center for Autoimmune Diseases, Sheba Medical Center, Tel-Aviv 
      University, Tel-Aviv, Israel.
FAU - Bashi, Tomer
AU  - Bashi T
FAU - Shoenfeld, Yehuda
AU  - Shoenfeld Y
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Immunoglobulin Idiotypes)
RN  - 0 (Immunoglobulins, Intravenous)
SB  - IM
MH  - Antibody Specificity/immunology
MH  - Autoimmune Diseases/therapy
MH  - *Chromatography, Affinity/methods
MH  - Humans
MH  - Immunoglobulin Idiotypes/immunology/*isolation & purification/therapeutic use
MH  - Immunoglobulins, Intravenous/immunology/*isolation & purification/therapeutic use
EDAT- 2013/09/17 06:00
MHDA- 2014/03/29 06:00
CRDT- 2013/09/17 06:00
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
AID - 10.1007/978-1-62703-586-6_18 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2014;1060:353-61. doi: 10.1007/978-1-62703-586-6_18.