PMID- 24038040
OWN - NLM
STAT- MEDLINE
DCOM- 20150409
LR  - 20161125
IS  - 1099-1263 (Electronic)
IS  - 0260-437X (Linking)
VI  - 34
IP  - 9
DP  - 2014 Sep
TI  - Proteomic profiling in incubation medium of mouse, rat and human precision-cut 
      liver slices for biomarker detection regarding acute drug-induced liver injury.
PG  - 993-1001
LID - 10.1002/jat.2917 [doi]
AB  - Drug-induced liver injury is one of the leading causes of drug withdrawal from 
      the market. In this study, we investigated the applicability of protein profiling 
      of the incubation medium of human, mouse and rat precision-cut liver slices 
      (PCLS) exposed to liver injury-inducing drugs for biomarker identification, using 
      matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. 
      PCLS were incubated with acetaminophen (APAP), 3-acetamidophenol, diclofenac and 
      lipopolysaccharide for 24-48 h. PCLS medium from all species treated with APAP 
      demonstrated similar changes in protein profiles, as previously found in mouse 
      urine after APAP-induced liver injury, including the same key proteins: 
      superoxide dismutase 1, carbonic anhydrase 3 and calmodulin. Further analysis 
      showed that the concentration of hepcidin, a hepatic iron-regulating hormone 
      peptide, was reduced in PCLS medium after APAP treatment, resembling the 
      decreased mouse plasma concentrations of hepcidin observed after APAP treatment. 
      Interestingly, comparable results were obtained after 3-acetamidophenol 
      incubation in rat and human, but not mouse PCLS. Incubation with diclofenac, but 
      not with lipopolysaccharide, resulted in the same toxicity parameters as observed 
      for APAP, albeit to a lesser extent. In conclusion, proteomics can be applied to 
      identify potential translational biomarkers using the PCLS system.
CI  - Copyright (c) 2013 John Wiley & Sons, Ltd.
FAU - van Swelm, Rachel P L
AU  - van Swelm RP
AD  - Department of Pharmacology and Toxicology, Radboud University Nijmegen Medical 
      Centre, the Netherlands.
FAU - Hadi, Mackenzie
AU  - Hadi M
FAU - Laarakkers, Coby M M
AU  - Laarakkers CM
FAU - Masereeuw, Rosalinde
AU  - Masereeuw R
FAU - Groothuis, Geny M M
AU  - Groothuis GM
FAU - Russel, Frans G M
AU  - Russel FG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130830
PL  - England
TA  - J Appl Toxicol
JT  - Journal of applied toxicology : JAT
JID - 8109495
RN  - 0 (Biomarkers)
RN  - 0 (Calmodulin)
RN  - 0 (Hepcidins)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (SOD1 protein, human)
RN  - 144O8QL0L1 (Diclofenac)
RN  - 362O9ITL9D (Acetaminophen)
RN  - EC 1.15.1.1 (Sod1 protein, mouse)
RN  - EC 1.15.1.1 (Sod1 protein, rat)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.15.1.1 (Superoxide Dismutase-1)
RN  - EC 4.2.1.- (Carbonic Anhydrase III)
SB  - IM
MH  - Acetaminophen/administration & dosage/toxicity
MH  - Animals
MH  - Biomarkers/*urine
MH  - Calmodulin/metabolism
MH  - Carbonic Anhydrase III/metabolism
MH  - Chemical and Drug Induced Liver Injury/*diagnosis
MH  - Diclofenac/administration & dosage/toxicity
MH  - *Gene Expression Profiling
MH  - Hepcidins/metabolism
MH  - Humans
MH  - Lipopolysaccharides/administration & dosage/toxicity
MH  - Liver/*drug effects/metabolism
MH  - Mice
MH  - *Proteomics
MH  - Rats
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Superoxide Dismutase/metabolism
MH  - Superoxide Dismutase-1
MH  - Toxicity Tests
OTO - NOTNLM
OT  - Precision-cut liver slices
OT  - acetaminophen
OT  - diclofenac
OT  - drug-induced liver injury
OT  - hepcidin
OT  - proteomics profiling
EDAT- 2013/09/17 06:00
MHDA- 2015/04/10 06:00
CRDT- 2013/09/17 06:00
PHST- 2013/05/27 00:00 [received]
PHST- 2013/06/21 00:00 [revised]
PHST- 2013/07/09 00:00 [accepted]
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2015/04/10 06:00 [medline]
AID - 10.1002/jat.2917 [doi]
PST - ppublish
SO  - J Appl Toxicol. 2014 Sep;34(9):993-1001. doi: 10.1002/jat.2917. Epub 2013 Aug 30.