PMID- 24038218
OWN - NLM
STAT- MEDLINE
DCOM- 20140422
LR  - 20211021
IS  - 1098-2825 (Electronic)
IS  - 0887-8013 (Print)
IS  - 0887-8013 (Linking)
VI  - 27
IP  - 5
DP  - 2013 Sep
TI  - A simple, fast correction method of triglyceride interference in blood hemoglobin 
      automated measurement.
PG  - 341-5
LID - 10.1002/jcla.21568 [doi]
AB  - BACKGROUND: To establish a reliable correction method for automated hemoglobin 
      (HGB) measurement by minimizing the interference from blood high triglyceride 
      (TG). METHODS: Fifty whole blood samples and 50 plasma samples containing 
      variable TG concentrations were used to determine the centrifugation speed and 
      time. Complete blood cell counts (CBCs) were performed by an automated hematology 
      analyzer for 102 blood samples, in which high-level TG were artificially added. 
      The same blood samples were centrifuged at low -speed to separate the plasma from 
      blood cells. Then the plasma was analyzed by the same analyzer. By using the two 
      CBC results, a correction formula was established to calculate the corrected HGB, 
      mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration 
      (MCHC) values. Comparisons were also made of HGB, MCH, and MCHC values before and 
      after correction of in-patient individuals who received intralipid and developed 
      lipemia. RESULTS: The percentage differences between the corrected and true 
      values of HGB, MCH and MCHC were -0.28%, 0.06%, and -0.31%, respectively. The 
      correlation coefficients of corrected values versus true values of HGB, MCH, and 
      MCHC were 0.989, 0.935, and 0.717, respectively. This correction method was also 
      effective for native lipemic samples. CONCLUSION: High blood TG level can cause 
      blood turbidity and erroneously high HGB results by hematology analyzers commonly 
      used in clinical laboratories. Adding a simple step of low-speed centrifugation 
      and measurement of HGB in the plasma fraction allows a quick correction of HGB 
      measurement in lipemic blood samples.
CI  - (c) 2012 Wiley Periodicals, Inc.
FAU - Zeng, Su-Gen
AU  - Zeng SG
AD  - Department of Laboratory Medicine, West China Hospital, Sichuan University, 
      Chengdu, P. R. China.
FAU - Zeng, Ting-Ting
AU  - Zeng TT
FAU - Jiang, Hong
AU  - Jiang H
FAU - Wang, Lan-Lan
AU  - Wang LL
FAU - Tang, Shu-Qiang
AU  - Tang SQ
FAU - Sun, Yu-Ming
AU  - Sun YM
FAU - Ying, Bin-Wu
AU  - Ying BW
FAU - Jia, Yong-Qian
AU  - Jia YQ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Clin Lab Anal
JT  - Journal of clinical laboratory analysis
JID - 8801384
RN  - 0 (Hemoglobins)
RN  - 0 (Triglycerides)
SB  - IM
MH  - *Automation, Laboratory
MH  - Blood Cell Count
MH  - Centrifugation
MH  - *Erythrocyte Indices
MH  - Hemoglobins/analysis
MH  - Humans
MH  - Hypertriglyceridemia/*blood
MH  - Triglycerides/*blood/chemistry
PMC - PMC6807499
OTO - NOTNLM
OT  - correction method
OT  - hemoglobin concentration
OT  - high triglyceride
EDAT- 2013/09/17 06:00
MHDA- 2014/04/23 06:00
PMCR- 2013/09/13
CRDT- 2013/09/17 06:00
PHST- 2012/07/29 00:00 [received]
PHST- 2012/11/05 00:00 [revised]
PHST- 2012/11/19 00:00 [accepted]
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2014/04/23 06:00 [medline]
PHST- 2013/09/13 00:00 [pmc-release]
AID - JCLA21568 [pii]
AID - 10.1002/jcla.21568 [doi]
PST - ppublish
SO  - J Clin Lab Anal. 2013 Sep;27(5):341-5. doi: 10.1002/jcla.21568.