PMID- 24040165
OWN - NLM
STAT- MEDLINE
DCOM- 20140615
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 9
DP  - 2013
TI  - Randomly detected genetically modified (GM) maize (Zea mays L.) near a transport 
      route revealed a fragile 45S rDNA phenotype.
PG  - e74060
LID - 10.1371/journal.pone.0074060 [doi]
LID - e74060
AB  - Monitoring of genetically modified (GM) crops has been emphasized to prevent 
      their potential effects on the environment and human health. Monitoring of the 
      inadvertent dispersal of transgenic maize in several fields and transport routes 
      in Korea was carried out by qualitative multiplex PCR, and molecular analyses 
      were conducted to identify the events of the collected GM maize. Cytogenetic 
      investigations through fluorescence in situ hybridization (FISH) of the GM maize 
      were performed to check for possible changes in the 45S rDNA cluster because this 
      cluster was reported to be sensitive to replication and transcription stress. 
      Three GM maize kernels were collected from a transport route near Incheon port, 
      Korea, and each was found to contain NK603, stacked MON863 x NK603, and stacked 
      NK603 x MON810 inserts, respectively. Cytogenetic analysis of the GM maize 
      containing the stacked NK603 x MON810 insert revealed two normal compact 5S rDNA 
      signals, but the 45S rDNA showed a fragile phenotype, demonstrating a 
      "beads-on-a-string" fragmentation pattern, which seems to be a consequence of 
      genetic modification. Implications of the 45S rDNA cluster fragility in GM maize 
      are also discussed.
FAU - Waminal, Nomar Espinosa
AU  - Waminal NE
AD  - Plant Biotechnology Institute, Department of Life Science, Sahmyook University, 
      Seoul, Korea ; Department of Plant Science, Plant Genomics and Breeding Institute 
      and Research Institute for Agriculture and Life Sciences, College of Agriculture 
      and Life Sciences, Seoul National University, Seoul, Korea.
FAU - Ryu, Ki Hyun
AU  - Ryu KH
FAU - Choi, Sun-Hee
AU  - Choi SH
FAU - Kim, Hyun Hee
AU  - Kim HH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130909
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Plant)
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Base Sequence
MH  - DNA, Plant/chemistry/*genetics
MH  - DNA, Ribosomal/chemistry/*genetics
MH  - Gene Expression
MH  - Gene Order
MH  - Genomic Instability
MH  - In Situ Hybridization, Fluorescence
MH  - Inheritance Patterns
MH  - Molecular Sequence Data
MH  - *Phenotype
MH  - Plants, Genetically Modified
MH  - Republic of Korea
MH  - Zea mays/*genetics/*metabolism
PMC - PMC3767626
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2013/09/17 06:00
MHDA- 2014/06/16 06:00
PMCR- 2013/09/09
CRDT- 2013/09/17 06:00
PHST- 2013/04/15 00:00 [received]
PHST- 2013/07/29 00:00 [accepted]
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2014/06/16 06:00 [medline]
PHST- 2013/09/09 00:00 [pmc-release]
AID - PONE-D-13-15754 [pii]
AID - 10.1371/journal.pone.0074060 [doi]
PST - epublish
SO  - PLoS One. 2013 Sep 9;8(9):e74060. doi: 10.1371/journal.pone.0074060. eCollection 
      2013.