PMID- 24040386
OWN - NLM
STAT- MEDLINE
DCOM- 20140701
LR  - 20211021
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 8
IP  - 9
DP  - 2013
TI  - Fish oil has beneficial effects on allergen-induced airway inflammation and 
      hyperreactivity in mice.
PG  - e75059
LID - 10.1371/journal.pone.0075059 [doi]
LID - e75059
AB  - BACKGROUND: Fish oil (FO) is rich in n-3 polyunsaturated fatty acids (PUFA), 
      which have been suggested to be anti-inflammatory and are associated with 
      improvement of several inflammatory diseases. In this study, we investigated the 
      influence of FO on allergen-induced lung inflammation and airway hyperreactivity 
      in mice. METHODS: Male A/J mice were fed either a standard-chow (SC) or a FO diet 
      (FO) for 8 weeks. After 4 weeks, each group was further randomized for ovalbumin 
      (SC-OVA and FO-OVA) or saline (SC-SAL and FO-SAL) challenge. Resistance and 
      elastance were measured at baseline and after aerosolized methacholine, 24h after 
      the last challenge. Bronchoalveolar lavage (BAL) was performed for leukocyte 
      counts. Lung tissue mucus deposition, peribronchiolar matrix deposition and 
      eosinophil infiltration were quantified. Serum immunoglobulin E (IgE) and IgG1 
      (ref 2.2), lung IL-4, IL-5, IL-10, IL-13, IL-17, INFgamma and eotaxin-1 and 2 were 
      detected by ELISA and nuclear factor kappa B (NFkappaB), GATA-3 and peroxisome 
      proliferator-activated receptor gamma (PPARgamma) expression was measured by Western 
      blot. RESULTS: Levels of serum IgE and IgG1 were significantly higher in OVA 
      sensitized mice. OVA challenge resulted in increased eosinophil infiltration, 
      increased inflammatory cytokine production, peribronchiolar matrix and mucus 
      deposition and airway hyperreactivity to aerosolized methacholine. Elevated lung 
      NFkappaB and GATA-3 expression was noted in OVA-challenged mice. These changes were 
      attenuated in mice fed with FO diet. Higher PPARgamma expression was also detected in 
      the lungs from the FO-fed groups. CONCLUSION: Our results demonstrate that FO 
      intake attenuated classical asthma features by suppressing the systemic 
      sensitization, thus providing evidence that FO might be a prophylactic 
      alternative for asthma prevention.
FAU - Bargut, Thereza Cristina Lonzetti
AU  - Bargut TC
AD  - Laboratory of Morphometry, Metabolism and Cardiovascular Disease, Biomedical 
      Center, Institute of Biology, State University of Rio de Janeiro, Rio de Janeiro, 
      Rio de Janeiro, Brazil.
FAU - Ferreira, Tatiana Paula Teixeira
AU  - Ferreira TP
FAU - Daleprane, Julio Beltrame
AU  - Daleprane JB
FAU - Martins, Marco Aurelio
AU  - Martins MA
FAU - Silva, Patricia Machado Rodrigues
AU  - Silva PM
FAU - Aguila, Marcia Barbosa
AU  - Aguila MB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130906
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Allergens)
RN  - 0 (Cytokines)
RN  - 0 (Fatty Acids, Unsaturated)
RN  - 0 (Fish Oils)
RN  - 0 (Immunoglobulin G)
RN  - 0W5ETF9M2K (Methacholine Chloride)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9006-59-1 (Ovalbumin)
RN  - EC 3.4.21.36 (Pancreatic Elastase)
SB  - IM
MH  - Allergens/*immunology
MH  - Animals
MH  - Bronchial Hyperreactivity/drug therapy/*pathology
MH  - Bronchoalveolar Lavage
MH  - Cytokines/metabolism
MH  - Fatty Acids, Unsaturated/metabolism
MH  - Fish Oils/*therapeutic use
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - Inflammation/drug therapy/*pathology
MH  - Leukocytes/drug effects
MH  - Lung/pathology
MH  - Male
MH  - Methacholine Chloride/immunology
MH  - Mice
MH  - Ovalbumin/immunology
MH  - Pancreatic Elastase/metabolism
PMC - PMC3765396
COIS- Competing Interests: MBA is currently a PLOS ONE Editorial Board member. This 
      does not alter the authors' adherence to all the PLOS ONE policies on sharing 
      data and materials.
EDAT- 2013/09/17 06:00
MHDA- 2014/07/02 06:00
PMCR- 2013/09/06
CRDT- 2013/09/17 06:00
PHST- 2012/12/26 00:00 [received]
PHST- 2013/08/12 00:00 [accepted]
PHST- 2013/09/17 06:00 [entrez]
PHST- 2013/09/17 06:00 [pubmed]
PHST- 2014/07/02 06:00 [medline]
PHST- 2013/09/06 00:00 [pmc-release]
AID - PONE-D-12-40692 [pii]
AID - 10.1371/journal.pone.0075059 [doi]
PST - epublish
SO  - PLoS One. 2013 Sep 6;8(9):e75059. doi: 10.1371/journal.pone.0075059. eCollection 
      2013.