PMID- 24041458 OWN - NLM STAT- MEDLINE DCOM- 20140519 LR - 20220419 IS - 1872-7573 (Electronic) IS - 0378-8741 (Linking) VI - 150 IP - 1 DP - 2013 Oct 28 TI - Neuroprotective effect of Liuwei Dihuang decoction on cognition deficits of diabetic encephalopathy in streptozotocin-induced diabetic rat. PG - 371-81 LID - S0378-8741(13)00636-3 [pii] LID - 10.1016/j.jep.2013.09.003 [doi] AB - ETHNOPHARMACOLOGICAL RELEVANCE: Liuwei Dihuang decoction (LWDHD) is a well-known prescription of traditional Chinese medicine (TCM) and consists of six crude drugs including Rehmannia glutinosa Libosch. (family: Scrophulariaceae), Cornus officinalis Sieb. (family: Cornaceae), Dioscorea oppositifolia L. (family: Dioscoreaceae), Paoenia ostii (family: Paeoniaceae), Alisma orientale (G. Samuelsson) Juz (family: Alismataceae) and Poria cocos (Schw.) Wolf (family: Polyporaceae). It has been used for the treatment of "Kidney-Yin" deficiency syndrome in clinic in China for a long time. Recent studies found that LWDHD had a potential benefit for the treatment of diabetic complications. The aim of the present study is to investigate the neuroprotective effect of LWDHD on memory and cognition deficits in streptozotocin (STZ)-induced diabetic encephalopathy (DE) rats. MATERIALS AND METHODS: Adult male Sprague Dawley (SD) rats were fed with high-glucose-fat diet for 50 days and then received an intraperitoneal injection of STZ (40 mg/kg) to induce DE model. Morris water maze test was used to evaluate the memory and cognition capability of DE rats. Choline acetyltransferase (ChAT), acetylcholinesterase (AChE), Na(+)-K(+)-ATP enzyme, iNOS and GSH kits were used to determine their activities or content in hippocampus. TUNEL staining, immunohistochemistry and Congo red staining were conducted to evaluate the apoptosis, caspase-3 protein expression, insulin-like growth factors 1 (IGF-1) and brain derived neurophic factor (BDNF) expressions, as well as Abeta deposition. RESULTS: The treatment with LWDHD (1 and 2g/kg, p.o., once daily, 30 days) could significantly reduce the escape latency time and path length, and obviously enhance the spent time in the target quadrant and platform crossings in Morris water maze test compared with model group (P<0.05, P<0.01). LWDHD could also significantly decrease the level of fasting blood glucose, increase Na(+)-K(+)-ATP enzyme and ChAT activities, enhance remarkedly GSH level while decrease significantly AChE and iNOS activities in hippocampus (P<0.05, P<0.01). Furthermore, TUNEL staining, Congo red staining and immunohistochemistry showed that LWDHD significantly improved the expressions of IGF-1 and BDNF, attenuated the neural apoptosis, overexpression of caspase-3 and Abeta deposition in the hippocampus and cerebral cortex of STZ-induced DE rats (P<0.01). CONCLUSION: Our findings suggested that LWDHD had a neuroprotective effect on DE rats. LWDHD may be of benefit in the treatment of DE. CI - (c) 2013 Elsevier Ireland Ltd. All rights reserved. FAU - Liu, Ji-ping AU - Liu JP AD - Department of Pharmacology of Chinese Materia Medica, China Pharmaceutical University, Jiangsu, Nanjing 210009, PR China; Department of Pharmacology, Shaanxi University of Chinese Medicine, Xianyang 712046, PR China. FAU - Feng, Liang AU - Feng L FAU - Zhang, Ming-hua AU - Zhang MH FAU - Ma, Dong-ying AU - Ma DY FAU - Wang, Shu-yuan AU - Wang SY FAU - Gu, Junfei AU - Gu J FAU - Fu, Qiang AU - Fu Q FAU - Qu, Rong AU - Qu R FAU - Ma, Shi-ping AU - Ma SP LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PT - Retracted Publication DEP - 20130914 PL - Ireland TA - J Ethnopharmacol JT - Journal of ethnopharmacology JID - 7903310 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Drugs, Chinese Herbal) RN - 0 (Liuwei Dihuang Decoction) RN - 0 (Neuroprotective Agents) RN - 67763-96-6 (Insulin-Like Growth Factor I) RN - EC 1.14.13.39 (Nitric Oxide Synthase Type II) RN - EC 1.14.13.39 (Nos2 protein, rat) RN - EC 2.3.1.6 (Choline O-Acetyltransferase) RN - EC 3.1.1.7 (Acetylcholinesterase) RN - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase) RN - GAN16C9B8O (Glutathione) SB - IM RIN - J Ethnopharmacol. 2022 Jun 12;291:115176. PMID: 35293313 MH - Acetylcholinesterase/metabolism MH - Animals MH - Brain-Derived Neurotrophic Factor/metabolism MH - Cerebral Cortex/drug effects/metabolism MH - Choline O-Acetyltransferase/metabolism MH - Cognition Disorders/*drug therapy/metabolism/physiopathology MH - Diabetes Mellitus, Experimental/complications/*drug therapy/metabolism/physiopathology MH - Drugs, Chinese Herbal/*therapeutic use MH - Glutathione/metabolism MH - Hippocampus/drug effects/metabolism MH - Insulin-Like Growth Factor I/metabolism MH - Male MH - Maze Learning/drug effects MH - Neuroprotective Agents/*therapeutic use MH - Nitric Oxide Synthase Type II/metabolism MH - Phytotherapy MH - Rats MH - Rats, Sprague-Dawley MH - Sodium-Potassium-Exchanging ATPase/metabolism OTO - NOTNLM OT - AChE OT - AD OT - ANOVA OT - Alzheimer's disease OT - Anti-apoptosis OT - Abeta OT - BDNF OT - ChAT OT - Cognition deficits OT - DAB OT - DE OT - DM OT - Diabetic encephalopathy OT - GSH OT - IGF-1 OT - LWDHD OT - Liuwei Dihuang decoction OT - NO OT - Na(+)-K(+)-ATPase OT - Na(+)-K(+)-adenosine triphosphatase OT - Neuropathological changes OT - OGD OT - Oxidative stress OT - ROS OT - SD OT - STZ OT - Sprague Dawley OT - TCM OT - TUNEL OT - acetylcholinesterase OT - amyloid beta OT - brain derived neurophic factor OT - choline acetyltransferase OT - diabetes mellitus OT - diabetic encephalopathy OT - diaminobenzidine OT - glutathione OT - insulin-like growth factors 1 OT - nitric oxide OT - one-way analysis of variance. OT - oxygen-glucose deprivation OT - reactive oxygen species OT - streptozotocin OT - terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling assay OT - traditional Chinese medicine EDAT- 2013/09/18 06:00 MHDA- 2014/05/20 06:00 CRDT- 2013/09/18 06:00 PHST- 2013/02/18 00:00 [received] PHST- 2013/09/02 00:00 [revised] PHST- 2013/09/05 00:00 [accepted] PHST- 2013/09/18 06:00 [entrez] PHST- 2013/09/18 06:00 [pubmed] PHST- 2014/05/20 06:00 [medline] AID - S0378-8741(13)00636-3 [pii] AID - 10.1016/j.jep.2013.09.003 [doi] PST - ppublish SO - J Ethnopharmacol. 2013 Oct 28;150(1):371-81. doi: 10.1016/j.jep.2013.09.003. Epub 2013 Sep 14.