PMID- 24041657
OWN - NLM
STAT- MEDLINE
DCOM- 20140318
LR  - 20131004
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 938
DP  - 2013 Nov 1
TI  - A rapid and highly sensitive UPLC-MS/MS method using pre-column derivatization 
      with 2-picolylamine for intravenous and percutaneous pharmacokinetics of valproic 
      acid in rats.
PG  - 35-42
LID - S1570-0232(13)00450-9 [pii]
LID - 10.1016/j.jchromb.2013.08.022 [doi]
AB  - A rapid, highly sensitive and specific ultra-performance liquid chromatography 
      coupled to tandem mass spectrometry (UPLC-MS/MS) for the detection of valproic 
      acid (VPA) in rat plasma following the topical application was developed and 
      validated. This method was carried out with pre-column derivatization using 
      2-picolylamine (PA) which reacts with the carboxylic acid group of VPA. The 
      derivatization was completed in 10min and the resulting PA-VPA derivative enabled 
      the sensitive detection of VPA in selected reaction monitoring (SRM) mode. Sample 
      preparation was done with simple liquid-liquid extraction and chromatographic 
      separation was achieved within 5min on a C18 column using a gradient elution with 
      the mobile phase of 2mM ammonium formate containing 0.1% formic acid and 
      methanol. The standard curves were linear over the concentration range of 
      0.07-200mug/mL with a correlation coefficient higher than 0.99. The limit of 
      detection (LOD) and the lower limit of quantification (LLOQ) was 0.03 and 
      0.07mug/mL, respectively with 100muL of plasma sample. The intra- and inter-day 
      precisions were measured to be below 10.7% and accuracies were within the range 
      of 94.1-115.9%. The validated method was successfully applied to the 
      pharmacokinetics of VPA in the rat following topical and intravenous 
      applications.
CI  - Copyright (c) 2013 Elsevier B.V. All rights reserved.
FAU - Joo, Kyung-Mi
AU  - Joo KM
AD  - AMOREPACIFIC CO/R&D Center, Gyeonggi-do 446-729, Republic of Korea.
FAU - Choi, Dalwoong
AU  - Choi D
FAU - Park, Yang-Hui
AU  - Park YH
FAU - Yi, Chang-Geun
AU  - Yi CG
FAU - Jeong, Hye-Jin
AU  - Jeong HJ
FAU - Cho, Jun-Cheol
AU  - Cho JC
FAU - Lim, Kyung-Min
AU  - Lim KM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130826
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 614OI1Z5WI (Valproic Acid)
SB  - IM
MH  - Administration, Intravenous
MH  - Administration, Topical
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Limit of Detection
MH  - Male
MH  - Nonlinear Dynamics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproducibility of Results
MH  - Tandem Mass Spectrometry/*methods
MH  - Valproic Acid/administration & dosage/*blood/chemistry/*pharmacokinetics
OTO - NOTNLM
OT  - 2-Picolylamine
OT  - Derivatization
OT  - Pharmacokinetics
OT  - Topical application
OT  - UPLC-MS/MS
OT  - Valproic acid
EDAT- 2013/09/18 06:00
MHDA- 2014/03/19 06:00
CRDT- 2013/09/18 06:00
PHST- 2013/03/20 00:00 [received]
PHST- 2013/07/10 00:00 [revised]
PHST- 2013/08/16 00:00 [accepted]
PHST- 2013/09/18 06:00 [entrez]
PHST- 2013/09/18 06:00 [pubmed]
PHST- 2014/03/19 06:00 [medline]
AID - S1570-0232(13)00450-9 [pii]
AID - 10.1016/j.jchromb.2013.08.022 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2013 Nov 1;938:35-42. doi: 
      10.1016/j.jchromb.2013.08.022. Epub 2013 Aug 26.