PMID- 24042200
OWN - NLM
STAT- MEDLINE
DCOM- 20131115
LR  - 20181202
IS  - 1554-6578 (Electronic)
IS  - 0022-3069 (Linking)
VI  - 72
IP  - 10
DP  - 2013 Oct
TI  - Blockade of interleukin 6 signaling improves the survival rate of transplanted 
      bone marrow stromal cells and increases locomotor function in mice with spinal 
      cord injury.
PG  - 980-93
LID - 10.1097/NEN.0b013e3182a79de9 [doi]
AB  - Bone marrow stromal cells (BMSCs) have the potential to improve functional 
      recovery in patients with spinal cord injury (SCI); however, they are limited by 
      low survival rates after transplantation in the injured tissue. Our objective was 
      to clarify the effects of a temporal blockade of interleukin 6 (IL-6)/IL-6 
      receptor (IL-6R) engagement using an anti-mouse IL-6R monoclonal antibody 
      (MR16-1) on the survival rate of BMSCs after their transplantation in a mouse 
      model of contusion SCI. MR16-1 cotreatment improved the survival rate of 
      transplanted BMSCs, allowing some BMSCs to differentiate into neurons and 
      astrocytes, and improved locomotor function recovery compared with BMSC 
      transplantation or MR16-1 treatment alone. The death of transplanted BMSCs could 
      be mainly related to apoptosis rather than necrosis. Transplantation of BMSC with 
      cotreatment of MR16-1 was associated with a decrease of some proinflammatory 
      cytokines, an increase of neurotrophic factors, decreased apoptosis rates of 
      transplanted BMSCs, and enhanced expression of survival factors Akt and 
      extracellular signal-regulated protein kinases 1/2. We conclude that MR16-1 
      treatment combined with BMSC transplants helped rescue neuronal cells and axons 
      after contusion SCI better than BMSCs alone by modulating the inflammatory/immune 
      responses and decreasing apoptosis.
FAU - Tan, Ying
AU  - Tan Y
AD  - From the Department of Spine Surgery, The First Affiliated Hospital, Sun Yat-Sen 
      University, Guangzhou, People's Republic of China (YT, SYL); Department of 
      Orthopaedics and Rehabilitation Medicine, Faculty of Medical Sciences, University 
      of Fukui, Fukui, Japan (YT, KU, HN, ARG, SW, TH, NT, HB); and Life and Health 
      Sciences, Aston University, Aston Triangle, Birmingham, United Kingdom (WEBJ).
FAU - Uchida, Kenzo
AU  - Uchida K
FAU - Nakajima, Hideaki
AU  - Nakajima H
FAU - Guerrero, Alexander R
AU  - Guerrero AR
FAU - Watanabe, Shuji
AU  - Watanabe S
FAU - Hirai, Takayuki
AU  - Hirai T
FAU - Takeura, Naoto
AU  - Takeura N
FAU - Liu, Shao-Yu
AU  - Liu SY
FAU - Johnson, William E B
AU  - Johnson WE
FAU - Baba, Hisatoshi
AU  - Baba H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Neuropathol Exp Neurol
JT  - Journal of neuropathology and experimental neurology
JID - 2985192R
RN  - 0 (Interleukin-6)
RN  - 0 (Receptors, Interleukin-6)
SB  - IM
MH  - Animals
MH  - Cell Survival/*drug effects/physiology
MH  - Disease Models, Animal
MH  - Interleukin-6/*antagonists & inhibitors
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Mesenchymal Stem Cells/*drug effects
MH  - Mice
MH  - Motor Activity/*drug effects/physiology
MH  - Neurons/drug effects/physiology
MH  - Receptors, Interleukin-6/*antagonists & inhibitors
MH  - Recovery of Function/drug effects/physiology
MH  - Signal Transduction/drug effects
MH  - Spinal Cord Injuries/*drug therapy/physiopathology/surgery
MH  - Survival Rate
EDAT- 2013/09/18 06:00
MHDA- 2013/11/16 06:00
CRDT- 2013/09/18 06:00
PHST- 2013/09/18 06:00 [entrez]
PHST- 2013/09/18 06:00 [pubmed]
PHST- 2013/11/16 06:00 [medline]
AID - 10.1097/NEN.0b013e3182a79de9 [doi]
PST - ppublish
SO  - J Neuropathol Exp Neurol. 2013 Oct;72(10):980-93. doi: 
      10.1097/NEN.0b013e3182a79de9.