PMID- 24043577
OWN - NLM
STAT- MEDLINE
DCOM- 20140619
LR  - 20220317
IS  - 1543-706X (Electronic)
IS  - 1071-2690 (Linking)
VI  - 49
IP  - 10
DP  - 2013 Dec
TI  - Human umbilical cord-derived MSC culture: the replacement of animal sera with 
      human cord blood plasma.
PG  - 771-7
LID - 10.1007/s11626-013-9663-8 [doi]
AB  - Human umbilical cord-derived mesenchymal stem cells (hUC-MSCs) hold great 
      potential for their therapeutic use in various clinical diseases. Many 
      publications have reported on human blood-derived alternatives to animal serum 
      for culturing mesenchymal stem cells, such as human serum, allogenic umbilical 
      cord blood serum, and human platelet derivatives. However, it is not clear 
      whether human umbilical cord blood plasma (UCBP), as the surplusage of umbilical 
      cord blood mesenchymal stem cell extraction, could be used. In this study, in 
      order to make the best of umbilical cord blood, the human UCBP was dialyzed to 
      replace fetal bovine serum (FBS) in the culture medium. hUC-MSCs were cultured in 
      the new medium. Cell growth rate, specific biomarkers, and differentiation 
      properties were detected to characterize the cell proliferation and MSC-specific 
      properties. The hUC-MSCs cultured in such derived medium were verified with 
      proliferation rate, cluster differentiation markers, cell cycle, as well as 
      differentiation capabilities. Such dialyzed human UCBP is fully comparable with, 
      if not superior to, FBS in deriving and culturing hUC-MSCs.
FAU - Ding, Yan
AU  - Ding Y
AD  - College of Life Science and Bioengineering, Beijing University of Technology, 
      Beijing, 100124, China.
FAU - Yang, Hua
AU  - Yang H
FAU - Feng, Jing Bo
AU  - Feng JB
FAU - Qiu, Ying
AU  - Qiu Y
FAU - Li, Dong Sheng
AU  - Li DS
FAU - Zeng, Yi
AU  - Zeng Y
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130917
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (DNA Primers)
SB  - IM
MH  - Cell Culture Techniques/*methods
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - DNA Primers/genetics
MH  - Fetal Blood
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/physiology
MH  - Plasma/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Umbilical Cord/*cytology
EDAT- 2013/09/18 06:00
MHDA- 2014/06/20 06:00
CRDT- 2013/09/18 06:00
PHST- 2013/04/03 00:00 [received]
PHST- 2013/07/07 00:00 [accepted]
PHST- 2013/09/18 06:00 [entrez]
PHST- 2013/09/18 06:00 [pubmed]
PHST- 2014/06/20 06:00 [medline]
AID - 10.1007/s11626-013-9663-8 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 2013 Dec;49(10):771-7. doi: 
      10.1007/s11626-013-9663-8. Epub 2013 Sep 17.