PMID- 24043764
OWN - NLM
STAT- MEDLINE
DCOM- 20131119
LR  - 20211021
IS  - 1540-9538 (Electronic)
IS  - 0022-1007 (Print)
IS  - 0022-1007 (Linking)
VI  - 210
IP  - 10
DP  - 2013 Sep 23
TI  - Intestinal monocytes and macrophages are required for T cell polarization in 
      response to Citrobacter rodentium.
PG  - 2025-39
LID - 10.1084/jem.20130903 [doi]
AB  - Dendritic cells (DCs), monocytes, and macrophages are closely related phagocytes 
      that share many phenotypic features and, in some cases, a common developmental 
      origin. Although the requirement for DCs in initiating adaptive immune responses 
      is well appreciated, the role of monocytes and macrophages remains largely 
      undefined, in part because of the lack of genetic tools enabling their specific 
      depletion. Here, we describe a two-gene approach that requires overlapping 
      expression of LysM and Csf1r to define and deplete monocytes and macrophages. The 
      role of monocytes and macrophages in immunity to pathogens was tested by their 
      selective depletion during infection with Citrobacter rodentium. Although neither 
      cell type was required to initiate immunity, monocytes and macrophages 
      contributed to the adaptive immune response by secreting IL-12, which induced Th1 
      polarization and IFN-gamma secretion. Thus, whereas DCs are indispensable for priming 
      naive CD4(+) T cells, monocytes and macrophages participate in intestinal 
      immunity by producing mediators that direct T cell polarization.
FAU - Schreiber, Heidi A
AU  - Schreiber HA
AD  - Laboratory of Molecular Immunology, 2 Mucosal Immunology and 3 Howard Hughes 
      Medical Institute, The Rockefeller University, New York, NY 10065.
FAU - Loschko, Jakob
AU  - Loschko J
FAU - Karssemeijer, Roos A
AU  - Karssemeijer RA
FAU - Escolano, Amelia
AU  - Escolano A
FAU - Meredith, Matthew M
AU  - Meredith MM
FAU - Mucida, Daniel
AU  - Mucida D
FAU - Guermonprez, Pierre
AU  - Guermonprez P
FAU - Nussenzweig, Michel C
AU  - Nussenzweig MC
LA  - eng
GR  - F32 AI096721/AI/NIAID NIH HHS/United States
GR  - P01 AI051573/AI/NIAID NIH HHS/United States
GR  - AI051573/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130916
PL  - United States
TA  - J Exp Med
JT  - The Journal of experimental medicine
JID - 2985109R
RN  - 187348-17-0 (Interleukin-12)
RN  - EC 2.7.10.1 (Receptor, Macrophage Colony-Stimulating Factor)
RN  - EC 3.2.1.17 (Muramidase)
SB  - IM
MH  - Adaptive Immunity
MH  - Animals
MH  - Cell Movement/immunology
MH  - Citrobacter rodentium/*immunology
MH  - Dendritic Cells/immunology/metabolism
MH  - Enterobacteriaceae Infections/genetics/immunology
MH  - Gene Order
MH  - Interleukin-12/biosynthesis/immunology
MH  - Intestines/*immunology/*microbiology
MH  - Macrophages/*immunology/metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Monocytes/*immunology/metabolism
MH  - Muramidase/genetics
MH  - Receptor, Macrophage Colony-Stimulating Factor/genetics/immunology
MH  - T-Lymphocyte Subsets/*immunology/metabolism
PMC - PMC3782042
EDAT- 2013/09/18 06:00
MHDA- 2013/11/20 06:00
PMCR- 2014/03/23
CRDT- 2013/09/18 06:00
PHST- 2013/09/18 06:00 [entrez]
PHST- 2013/09/18 06:00 [pubmed]
PHST- 2013/11/20 06:00 [medline]
PHST- 2014/03/23 00:00 [pmc-release]
AID - jem.20130903 [pii]
AID - 20130903 [pii]
AID - 10.1084/jem.20130903 [doi]
PST - ppublish
SO  - J Exp Med. 2013 Sep 23;210(10):2025-39. doi: 10.1084/jem.20130903. Epub 2013 Sep 
      16.