PMID- 24044225
OWN - NLM
STAT- MEDLINE
DCOM- 20131114
LR  - 20130918
IS  - 1003-9279 (Print)
IS  - 1003-9279 (Linking)
VI  - 27
IP  - 2
DP  - 2013 Apr
TI  - [Establishment and clinical application of TapMan real-time RT-PCR method for 
      detection of HHV-6].
PG  - 144-6
AB  - OBJECTIVE: To establish a rapid, sensitive and specific real-time PCR method for 
      detection of Human Herpesvirus-6 (HHV-6). METHODS: According to the reference, a 
      pair of primers and a probe were designed located in U65-66 gene and to set up 
      the standards. We established a real-time RT-PCR method for detection of HHV-6, 
      and to verify the specificity, sensitivity, reproducibility. RESULTS: The 
      correlation coefficient was 0.999, E = 97.9%, the coefficient of variation values 
      of Ct were 0.61% and 3.13% in real-time PCR assay for inter and intra assay, 
      respectively. The results of all viruses were negative except of HHV-6 for the 
      assay. The quantitative detection limit of the assay was 3 x 10(0) copies/microl. 
      CONCLUSION: The real-time PCR assay is highly specific, sensitive and 
      reproducible, which can be used to quatitative detecting clinical samples.
FAU - Chen, Qian-Qian
AU  - Chen QQ
AD  - University of South China, Hengyang 421000, China.
FAU - Zhang, Bing
AU  - Zhang B
FAU - Xie, Zhi-Ping
AU  - Xie ZP
FAU - Li, Jin-Song
AU  - Li JS
FAU - Gao, Han-Chun
AU  - Gao HC
FAU - Xiao, Ni-Guang
AU  - Xiao NG
FAU - Xie, Le-Yun
AU  - Xie LY
FAU - Yu, Tian
AU  - Yu T
FAU - Zeng, Sai-Zhen
AU  - Zeng SZ
FAU - Gong, Ping
AU  - Gong P
FAU - Duan, Zhao-Jun
AU  - Duan ZJ
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
JT  - Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang 
      bingduxue zazhi = Chinese journal of experimental and clinical virology
JID - 9602873
SB  - IM
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Reproducibility of Results
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
EDAT- 2013/09/21 06:00
MHDA- 2013/11/15 06:00
CRDT- 2013/09/19 06:00
PHST- 2013/09/19 06:00 [entrez]
PHST- 2013/09/21 06:00 [pubmed]
PHST- 2013/11/15 06:00 [medline]
PST - ppublish
SO  - Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Apr;27(2):144-6.