PMID- 24045481
OWN - NLM
STAT- MEDLINE
DCOM- 20140623
LR  - 20131031
IS  - 1525-3163 (Electronic)
IS  - 0021-8812 (Linking)
VI  - 91
IP  - 11
DP  - 2013 Nov
TI  - Effect of nutrition on plasma lipid profile and mRNA levels of ovarian genes 
      involved in steroid hormone synthesis in Hu sheep during luteal phase.
PG  - 5229-39
LID - 10.2527/jas.2013-6450 [doi]
AB  - Ovarian steroid hormones regulate follicular growth and atresia. This study aims 
      to determine whether key ovarian sterol-regulatory genes are differentially 
      expressed in Hu sheep under different short-term nutritional regimens. Estrus was 
      synchronized using intravaginal progestagen sponges. The ewes were assigned 
      randomly to 3 groups. On d 6 to 12 of their estrous cycle, the control (CON) 
      group received a maintenance diet (1.0xM), the supplemented (SUP) group received 
      1.5xM, and the restricted (R) group received 0.5xM. On d 7 to 12, blood samples 
      were taken. The sheep were slaughtered at the end of the treatment, and their 
      organs and ovaries were collected. The plasma concentrations of urea (P<0.01), 
      total cholesterol (P<0.01), low-density lipoprotein cholesterol (P<0.01), NEFA 
      (P<0.01), FSH (P<0.05), and estradiol (P<0.05) increased with decreasing dietary 
      intake, whereas plasma triglyceride (P<0.01) and triiodothyronine (T3) 
      concentrations decreased (P<0.05). The ewes in the R group had higher spleen 
      weight and percentage of spleen to BW and lower liver and small intestine weights 
      and percentage of liver/stomach to BW than the SUP group ewes (P<0.05). 
      Nutritional restriction decreased the cytochrome p450 (CYP17A1) and estrogen 
      receptor 1 (ESR1) mRNA expression (P<0.05) and increased the cytochrome p450 
      aromatase (CYP19A1) mRNA expression (P<0.05) in follicles>2.5 mm. Follicle size 
      affected the mRNA expression of very low density lipoprotein receptor (VLDLR), 
      estrogen receptor 2 (ESR2), FSH receptor (FSHR), CYP17A1, and CYP19A1 (P<0.05). 
      In conclusion, we suggest that a potential mechanism by which short-term negative 
      energy balance inhibits follicular growth may involve responses to disrupted 
      reproductive hormone concentrations and influenced the intrafollicular expression 
      of CYP17A1, CYP19A1, and ESR1. This result may be due to increased plasma urea 
      and lipid concentrations.
FAU - Ying, S J
AU  - Ying SJ
AD  - Jiangsu Engineering Technology Research Center of Meat Sheep and Goat Industry, 
      Nanjing Agricultural University, Nanjing 210095, Jiangsu Province, China.
FAU - Xiao, S H
AU  - Xiao SH
FAU - Wang, C L
AU  - Wang CL
FAU - Zhong, B S
AU  - Zhong BS
FAU - Zhang, G M
AU  - Zhang GM
FAU - Wang, Z Y
AU  - Wang ZY
FAU - He, D Y
AU  - He DY
FAU - Ding, X L
AU  - Ding XL
FAU - Xing, H J
AU  - Xing HJ
FAU - Wang, F
AU  - Wang F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130917
PL  - United States
TA  - J Anim Sci
JT  - Journal of animal science
JID - 8003002
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (Lipids)
RN  - 0 (Progestins)
RN  - 0 (RNA, Messenger)
RN  - 8W8T17847W (Urea)
SB  - IM
MH  - Animal Feed
MH  - Animal Nutritional Physiological Phenomena
MH  - Animals
MH  - Body Weight
MH  - Diet/veterinary
MH  - Estrus Synchronization
MH  - Female
MH  - Gene Expression Regulation/*physiology
MH  - Gonadal Steroid Hormones/*biosynthesis
MH  - Lipids/*blood
MH  - Luteal Phase/physiology
MH  - *Nutritional Status
MH  - Ovary/physiology
MH  - Progestins/administration & dosage/pharmacology
MH  - RNA, Messenger/genetics/*metabolism
MH  - Sheep/blood/*genetics/metabolism
MH  - Urea/blood
EDAT- 2013/09/21 06:00
MHDA- 2014/06/24 06:00
CRDT- 2013/09/19 06:00
PHST- 2013/09/19 06:00 [entrez]
PHST- 2013/09/21 06:00 [pubmed]
PHST- 2014/06/24 06:00 [medline]
AID - jas.2013-6450 [pii]
AID - 10.2527/jas.2013-6450 [doi]
PST - ppublish
SO  - J Anim Sci. 2013 Nov;91(11):5229-39. doi: 10.2527/jas.2013-6450. Epub 2013 Sep 
      17.