PMID- 24050301
OWN - NLM
STAT- MEDLINE
DCOM- 20140401
LR  - 20171116
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 61
IP  - 41
DP  - 2013 Oct 16
TI  - Mulberry 1-deoxynojirimycin pleiotropically inhibits glucose-stimulated vascular 
      smooth muscle cell migration by activation of AMPK/RhoB and down-regulation of 
      FAK.
PG  - 9867-75
LID - 10.1021/jf403636z [doi]
AB  - Mulberry 1-deoxynojirimycin (DNJ), an inhibitor of alpha-glucosidase, has been 
      reported to help prevent diabetes mellitus and suppress lipid accumulation. The 
      aim of this study was to determine whether mulberry DNJ has pleiotropic effects 
      on the development of atherosclerosis. The mechanisms by which mulberry DNJ might 
      inhibit migration of A7r5 vascular smooth muscle cells (VSMCs) under 
      hyperglycemic conditions mimicking diabetes were investigated. The antimigratory 
      effects of DNJ on VSMCs were assessed by Western blot analysis of 
      migration-related proteins and by electric cell-substrate impedance sensing 
      (ECIS) and visualization of F-actin cytoskeleton. Two pathways of DNJ-mediated 
      inhibition of VSMC migration were identified. The first involved AMPK activation 
      to inhibit fatty acid synthase (FASN) and Akt activity and then RhoB activation 
      to inhibit nuclear factor-kappaB (NF-kappaB) and matrix metalloproteinase-2 (MMP) 
      activity. The second involved inhibition of focal adhesion kinase (FAK), Ras, and 
      RhoA activity leading to inhibition of F-actin activity.
FAU - Chan, Kuei-Chuan
AU  - Chan KC
AD  - Department of Internal Medicine, Chung-Shan Medical University Hospital , No. 
      110, Sec. 1, Jianguo N. Road, Taichung 402, Taiwan.
FAU - Lin, Ming-Cheng
AU  - Lin MC
FAU - Huang, Chien-Ning
AU  - Huang CN
FAU - Chang, Wen-Chun
AU  - Chang WC
FAU - Wang, Chau-Jong
AU  - Wang CJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20131002
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Plant Extracts)
RN  - 19130-96-2 (1-Deoxynojirimycin)
RN  - EC 2.7.10.2 (Focal Adhesion Protein-Tyrosine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 3.6.5.2 (rhoB GTP-Binding Protein)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - 1-Deoxynojirimycin/*pharmacology
MH  - AMP-Activated Protein Kinases/*genetics/metabolism
MH  - Animals
MH  - Atherosclerosis/drug therapy/enzymology/genetics/*physiopathology
MH  - Cell Line
MH  - Cell Movement/drug effects
MH  - Down-Regulation/drug effects
MH  - Enzyme Activation/drug effects
MH  - Focal Adhesion Protein-Tyrosine Kinases/*genetics/metabolism
MH  - Glucose/*metabolism
MH  - Humans
MH  - Morus/*chemistry
MH  - Muscle, Smooth, Vascular/cytology/*drug effects/enzymology/metabolism
MH  - Plant Extracts/*pharmacology
MH  - Rats
MH  - Signal Transduction/drug effects
MH  - rhoB GTP-Binding Protein/genetics/metabolism
EDAT- 2013/09/21 06:00
MHDA- 2014/04/02 06:00
CRDT- 2013/09/21 06:00
PHST- 2013/09/21 06:00 [entrez]
PHST- 2013/09/21 06:00 [pubmed]
PHST- 2014/04/02 06:00 [medline]
AID - 10.1021/jf403636z [doi]
PST - ppublish
SO  - J Agric Food Chem. 2013 Oct 16;61(41):9867-75. doi: 10.1021/jf403636z. Epub 2013 
      Oct 2.