PMID- 24050774
OWN - NLM
STAT- MEDLINE
DCOM- 20140428
LR  - 20180305
IS  - 1476-4245 (Electronic)
IS  - 1475-4916 (Linking)
VI  - 102
IP  - 4
DP  - 2013 Oct
TI  - Anti-proliferative effects of homeopathic medicines on human kidney, colon and 
      breast cancer cells.
PG  - 274-82
LID - S1475-4916(13)00041-6 [pii]
LID - 10.1016/j.homp.2013.06.001 [doi]
AB  - OBJECTIVE: Homeopathy is controversial, due to the claims made for very high 
      dilutions. Although several theories are proposed to understand the mechanisms of 
      action, none are scientifically verified. This study aimed to investigate the 
      efficacy of the selected homeopathic medicines in specific in vitro cancer 
      models. METHODS: We assessed the cytotoxic activity of selected homeopathic 
      medicines in mother tincture (MT), and ultramolecular dilution (30C, 200C, 1M and 
      10M) against cell lines deriving from tumors of particular organs, Sarsaparilla 
      (Sars) on ACHN cells (human renal adenocarcinoma), Ruta graveolens (Ruta) on 
      COLO-205 (human colorectal carcinoma), and Phytolacca decandra (Phyto) on MCF-7 
      (human breast carcinoma). Sars was also tested against Madin-Darby canine kidney 
      (MDCK) cells (a non-malignant cell line). Cytotoxicity was measured using the 
      3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide (MTT) method, 
      anti-proliferative activity by trypan blue exclusion assay, apoptosis determined 
      by dual staining the cells with ethidium bromide (EB) and acridine orange (AO) 
      dyes. RESULTS: MTs and ultra-diluted preparations of the three homeopathic 
      medicines had highly significant effects in the respective cancer cell lines, 
      producing cytotoxicity and a decrease in cell proliferation. The effects were 
      greatest with the MTs, but in all cases and persisted, although to a lesser 
      degree in the ultra-diluted molecular preparations. Sars showed no effect on MDCK 
      cells. In the homeopathic medicine treated cultures, hallmarks of apoptosis were 
      evident including, cell shrinkage, chromatin condensation and DNA fragmentation. 
      CONCLUSION: This study provides preliminary laboratory evidence indicating the 
      ability of homeopathic medicines as anticancer agents. Further studies of the 
      action of these homeopathic remedies are warranted.
CI  - Copyright (c) 2013 The Faculty of Homeopathy. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Arora, Shagun
AU  - Arora S
AD  - Jaypee University of Information Technology, Waknaghat, Solan 173234, Himachal 
      Pradesh, India.
FAU - Aggarwal, Ayushi
AU  - Aggarwal A
FAU - Singla, Priyanka
AU  - Singla P
FAU - Jyoti, Saras
AU  - Jyoti S
FAU - Tandon, Simran
AU  - Tandon S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Homeopathy
JT  - Homeopathy : the journal of the Faculty of Homeopathy
JID - 101140517
RN  - 0 (Antineoplastic Agents)
RN  - 0 (Cytotoxins)
RN  - 0 (Plant Preparations)
SB  - IM
MH  - Animals
MH  - Antineoplastic Agents/*pharmacology
MH  - Apoptosis/*drug effects
MH  - Breast Neoplasms/*drug therapy/pathology
MH  - Cell Line, Tumor/drug effects
MH  - Cell Proliferation/drug effects
MH  - Colonic Neoplasms/*drug therapy/pathology
MH  - Cytotoxins/pharmacology
MH  - Dogs
MH  - Female
MH  - *Homeopathy/methods
MH  - Humans
MH  - Kidney Neoplasms/*drug therapy/pathology
MH  - Phytolacca dodecandra
MH  - Phytotherapy
MH  - Plant Preparations/*pharmacology
MH  - Ruta
MH  - Smilax
OTO - NOTNLM
OT  - Anti-proliferative
OT  - Apoptosis
OT  - Cancer
OT  - Cell culture
OT  - Cytotoxicity
OT  - Homeopathy
OT  - Phytolacca decandra
OT  - Ruta graveolens
OT  - Sarsaparilla
EDAT- 2013/09/21 06:00
MHDA- 2014/04/29 06:00
CRDT- 2013/09/21 06:00
PHST- 2013/02/15 00:00 [received]
PHST- 2013/05/02 00:00 [revised]
PHST- 2013/06/21 00:00 [accepted]
PHST- 2013/09/21 06:00 [entrez]
PHST- 2013/09/21 06:00 [pubmed]
PHST- 2014/04/29 06:00 [medline]
AID - S1475-4916(13)00041-6 [pii]
AID - 10.1016/j.homp.2013.06.001 [doi]
PST - ppublish
SO  - Homeopathy. 2013 Oct;102(4):274-82. doi: 10.1016/j.homp.2013.06.001.