PMID- 24051200
OWN - NLM
STAT- MEDLINE
DCOM- 20131223
LR  - 20161125
IS  - 1872-8421 (Electronic)
IS  - 0165-5728 (Linking)
VI  - 264
IP  - 1-2
DP  - 2013 Nov 15
TI  - Immunoglobulinfree light chains reduce in an antigen-specific manner the rate of 
      rise of action potentials of mouse non-nociceptive dorsal root ganglion neurons.
PG  - 14-23
LID - S0165-5728(13)00238-5 [pii]
LID - 10.1016/j.jneuroim.2013.08.015 [doi]
AB  - Plasma B cells secrete immunoglobulinfree light chains (IgLC) which by binding to 
      mast cells can mediate hypersensitivity responses and are involved in several 
      immunological disorders. To investigate the effects of antigen-specific IgLC 
      activation, intracellular recordings were made from cultured murine dorsal root 
      ganglion (DRG) neurons, which can specifically bind IgLC. The neurons were 
      sensitized with IgLC for 90min and subsequently activated by application of the 
      corresponding antigen (DNP-HSA). Antigen application induced a decrease in the 
      rate of rise of the action potentials of non-nociceptive neurons (MANOVA, 
      p=2.10(-6)), without affecting the resting membrane potential or firing 
      threshold. The action potentials of the nociceptive neurons (p=0.57) and the 
      electrical excitability of both types of neurons (p>0.35) were not affected. We 
      conclude that IgLC can mediate antigen-specific responses by reducing the rate of 
      rise of action potentials in non-nociceptive murine DRG neurons. We suggest that 
      antigen-specific activation of IgLC-sensitized non-nociceptive DRG neurons may 
      contribute to immunological hypersensitivity responses and neuroinflammation.
CI  - (c) 2013.
FAU - Rijnierse, Anneke
AU  - Rijnierse A
AD  - Division of Pharmacology & Pathophysiology, Utrecht Institute for Pharmaceutical 
      Sciences, Faculty of Science, Utrecht University, Utrecht, The Netherlands. 
      Electronic address: anneke.rijnierse@danone.com.
FAU - Kraneveld, Aletta D
AU  - Kraneveld AD
FAU - Salemi, Arezo
AU  - Salemi A
FAU - Zwaneveld, Sandra
AU  - Zwaneveld S
FAU - Goumans, Aleida P H
AU  - Goumans AP
FAU - Rychter, Jakub W
AU  - Rychter JW
FAU - Thio, Marco
AU  - Thio M
FAU - Redegeld, Frank A
AU  - Redegeld FA
FAU - Westerink, Remco H S
AU  - Westerink RH
FAU - Kroese, Alfons B A
AU  - Kroese AB
LA  - eng
PT  - Journal Article
DEP - 20130831
PL  - Netherlands
TA  - J Neuroimmunol
JT  - Journal of neuroimmunology
JID - 8109498
RN  - 0 (Antigens)
RN  - 0 (Immunoglobulin Light Chains)
SB  - IM
MH  - Action Potentials/*drug effects
MH  - Analysis of Variance
MH  - Animals
MH  - Antigens/metabolism
MH  - Biophysical Phenomena/drug effects
MH  - Biophysics
MH  - Cells, Cultured
MH  - Electric Stimulation
MH  - Ganglia, Spinal/*cytology
MH  - Immunoglobulin Light Chains/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Neurons/classification/*drug effects/physiology
MH  - Time Factors
OTO - NOTNLM
OT  - Action potential duration
OT  - Hypersensitivity reaction
OT  - Na(v) channel
OT  - Neuroinflammation
OT  - Nociceptive neurons
OT  - Pain
EDAT- 2013/09/21 06:00
MHDA- 2013/12/24 06:00
CRDT- 2013/09/21 06:00
PHST- 2013/01/08 00:00 [received]
PHST- 2013/08/16 00:00 [revised]
PHST- 2013/08/26 00:00 [accepted]
PHST- 2013/09/21 06:00 [entrez]
PHST- 2013/09/21 06:00 [pubmed]
PHST- 2013/12/24 06:00 [medline]
AID - S0165-5728(13)00238-5 [pii]
AID - 10.1016/j.jneuroim.2013.08.015 [doi]
PST - ppublish
SO  - J Neuroimmunol. 2013 Nov 15;264(1-2):14-23. doi: 10.1016/j.jneuroim.2013.08.015. 
      Epub 2013 Aug 31.