PMID- 24051401
OWN - NLM
STAT- MEDLINE
DCOM- 20140407
LR  - 20211203
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 14
IP  - 9
DP  - 2013 Sep 18
TI  - Involvement of calcium-mediated reactive oxygen species in inductive GRP78 
      expression by geldanamycin in 9L rat brain tumor cells.
PG  - 19169-85
LID - 10.3390/ijms140919169 [doi]
AB  - Treatment with geldanamycin (GA) leads to an increase in [Ca2+]c and the 
      production of reactive oxygen species (ROS) in rat brain tumor 9L RBT cells. 
      GA-exerted calcium signaling was blocked by BAPTA/AM and EGTA. The effect of GA 
      on [Ca2+]c was significantly reduced in the presence of thapsigargin (TG) and 
      ruthenium red (RR). GA-induced GRP78 expression is significantly decreased in the 
      presence of BAPTA/AM, EGTA and RR, suggesting that the calcium influx from the 
      extracellular space and intracellular calcium store oscillations are contributed 
      to by the calcium mobilization and GRP78 expression induced by GA. The induced 
      GRP78 expression is sensitive to added U73122 and Ro-31-8425, pinpointing the 
      involvement of phospholipase C (PLC) and protein kinase C (PKC) in GA-induced 
      endoplasmic reticulum (ER) stress. The antioxidants N-acetylcysteine (NAC), 
      BAPTA/AM, EGTA and H7 also have significant inhibitory effects on ROS generation. 
      Finally, neither H7 nor NAC was able to affect the calcium response elicited by 
      GA. Our results suggest that the causal signaling cascade during GA-inducted 
      GRP78 expression occurs via a pathway that connects PLC to cytoplasmic calcium 
      increase, PKC activation and, then, finally, ROS generation. Our data provides 
      new insights into the influence of GA on ER stress response in 9L RBT cells.
FAU - Sun, Fang-Chun
AU  - Sun FC
AD  - Department of Bioresources, Da-Yeh University, Changhua 515, Taiwan. 
      fcsun@mail.dyu.edu.tw.
FAU - Shyu, Hsin-Yi
AU  - Shyu HY
FAU - Lee, Meng-Shiou
AU  - Lee MS
FAU - Lee, Meng-Shiunn
AU  - Lee MS
FAU - Lai, Yiu-Kay
AU  - Lai YK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130918
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Antibiotics, Antineoplastic)
RN  - 0 (Benzoquinones)
RN  - 0 (Chelating Agents)
RN  - 0 (Endoplasmic Reticulum Chaperone BiP)
RN  - 0 (Estrenes)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (Indoles)
RN  - 0 (Lactams, Macrocyclic)
RN  - 0 (Maleimides)
RN  - 0 (Pyrrolidinones)
RN  - 0 (Reactive Oxygen Species)
RN  - 112648-68-7 
      (1-(6-((3-methoxyestra-1,3,5(10)-trien-17-yl)amino)hexyl)-1H-pyrrole-2,5-dione)
RN  - 131848-97-0 (Ro 31-8425)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 3.1.4.- (Type C Phospholipases)
RN  - SY7Q814VUP (Calcium)
RN  - Z3K3VJ16KU (geldanamycin)
SB  - IM
MH  - Animals
MH  - Antibiotics, Antineoplastic/*toxicity
MH  - Benzoquinones/*toxicity
MH  - Brain Neoplasms/metabolism/pathology
MH  - Calcium/*metabolism
MH  - Cell Line, Tumor
MH  - Chelating Agents/pharmacology
MH  - Endoplasmic Reticulum Chaperone BiP
MH  - Endoplasmic Reticulum Stress/drug effects
MH  - Estrenes/pharmacology
MH  - Gene Expression/*drug effects
MH  - Heat-Shock Proteins/genetics/*metabolism
MH  - Indoles/pharmacology
MH  - Lactams, Macrocyclic/*toxicity
MH  - Maleimides/pharmacology
MH  - Protein Kinase C/metabolism
MH  - Pyrrolidinones/pharmacology
MH  - Rats
MH  - Reactive Oxygen Species/*metabolism
MH  - Type C Phospholipases/antagonists & inhibitors/metabolism
PMC - PMC3794827
EDAT- 2013/09/21 06:00
MHDA- 2014/04/08 06:00
PMCR- 2013/09/01
CRDT- 2013/09/21 06:00
PHST- 2013/07/24 00:00 [received]
PHST- 2013/08/19 00:00 [revised]
PHST- 2013/09/09 00:00 [accepted]
PHST- 2013/09/21 06:00 [entrez]
PHST- 2013/09/21 06:00 [pubmed]
PHST- 2014/04/08 06:00 [medline]
PHST- 2013/09/01 00:00 [pmc-release]
AID - ijms140919169 [pii]
AID - ijms-14-19169 [pii]
AID - 10.3390/ijms140919169 [doi]
PST - epublish
SO  - Int J Mol Sci. 2013 Sep 18;14(9):19169-85. doi: 10.3390/ijms140919169.