PMID- 24052228
OWN - NLM
STAT- MEDLINE
DCOM- 20140520
LR  - 20211021
IS  - 1476-5535 (Electronic)
IS  - 1367-5435 (Linking)
VI  - 40
IP  - 12
DP  - 2013 Dec
TI  - Cloning, expression of a feruloyl esterase from Aspergillus usamii E001 and its 
      applicability in generating ferulic acid from wheat bran.
PG  - 1433-41
LID - 10.1007/s10295-013-1339-6 [doi]
AB  - A cDNA gene (AufaeA), which encodes a mature polypeptide of the type-A feruloyl 
      esterase from Aspergillus usamii E001 (abbreviated to AuFaeA), was cloned and 
      heterologously expressed in Pichia pastoris GS115. One transformant, labeled as 
      P. pastoris GSFaeA4-8, expressing the highest recombinant AuFaeA (reAuFaeA) 
      activity of 10.76 U/ml was selected by the flask expression test. The expressed 
      reAuFaeA was purified to homogeneity with an apparent molecular weight of 
      36.0 kDa by SDS-PAGE analysis, and characterized using the model substrate of 
      methyl ferulate (MFA). The purified reAuFaeA was optimally active at pH 5.0 and 
      45  degrees C, and highly stable at pH 4.0-6.5 and 45  degrees C or below. Its activity was not 
      significantly affected by metal ions tested and EDTA. The K m and V max of 
      reAuFaeA towards MFA were 4.64 mM and 115.5 U/mg, respectively. High-performance 
      liquid chromatography analysis showed that only 9.7 % of total alkali-extractable 
      ferulic acid (FA) was released from destarched wheat bran by reAuFaeA alone. The 
      released FA increased to 36.5 % when reAuFaeA was used together with a 
      recombinant Aspergillus usamii GH family 11 xylanase A, indicating a synergistic 
      interaction between them.
FAU - Gong, Yan-Yan
AU  - Gong YY
AD  - School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Road, Wuxi, 
      214122, Jiangsu, People's Republic of China.
FAU - Yin, Xin
AU  - Yin X
FAU - Zhang, Hui-Min
AU  - Zhang HM
FAU - Wu, Min-Chen
AU  - Wu MC
FAU - Tang, Cun-Duo
AU  - Tang CD
FAU - Wang, Jun-Qing
AU  - Wang JQ
FAU - Pang, Qing-Feng
AU  - Pang QF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130920
PL  - Germany
TA  - J Ind Microbiol Biotechnol
JT  - Journal of industrial microbiology & biotechnology
JID - 9705544
RN  - 0 (Coumaric Acids)
RN  - 0 (DNA, Complementary)
RN  - 0 (Dietary Fiber)
RN  - 0 (Fungal Proteins)
RN  - 0 (Recombinant Proteins)
RN  - AVM951ZWST (ferulic acid)
RN  - EC 3.1.1.- (Carboxylic Ester Hydrolases)
RN  - EC 3.1.1.73 (feruloyl esterase)
RN  - EC 3.2.1.8 (Endo-1,4-beta Xylanases)
SB  - IM
MH  - Amino Acid Sequence
MH  - Aspergillus/*enzymology/genetics
MH  - Carboxylic Ester Hydrolases/genetics/isolation & purification/*metabolism
MH  - Cloning, Molecular
MH  - Computational Biology
MH  - Coumaric Acids/*metabolism
MH  - DNA, Complementary/metabolism
MH  - Dietary Fiber/*metabolism
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Endo-1,4-beta Xylanases/metabolism
MH  - Fungal Proteins/genetics/isolation & purification/*metabolism
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Pichia/enzymology/genetics
MH  - Polymerase Chain Reaction
MH  - Protein Conformation
MH  - Recombinant Proteins/genetics/metabolism
MH  - Sequence Homology, Amino Acid
EDAT- 2013/09/21 06:00
MHDA- 2014/05/21 06:00
CRDT- 2013/09/21 06:00
PHST- 2013/05/25 00:00 [received]
PHST- 2013/09/02 00:00 [accepted]
PHST- 2013/09/21 06:00 [entrez]
PHST- 2013/09/21 06:00 [pubmed]
PHST- 2014/05/21 06:00 [medline]
AID - 10.1007/s10295-013-1339-6 [doi]
PST - ppublish
SO  - J Ind Microbiol Biotechnol. 2013 Dec;40(12):1433-41. doi: 
      10.1007/s10295-013-1339-6. Epub 2013 Sep 20.