PMID- 24053955
OWN - NLM
STAT- MEDLINE
DCOM- 20141113
LR  - 20130923
IS  - 1001-9391 (Print)
IS  - 1001-9391 (Linking)
VI  - 31
IP  - 8
DP  - 2013 Aug
TI  - [DNA damage of splenic lymphocytes in pregnant mice exposed to carbon disulfide 
      in implantation phase].
PG  - 576-80
AB  - OBJECTIVE: To investigate the DNA damage of splenic lymphocytes in pregnant mice 
      exposed to carbon disulfide (CS2) in the implantation phase and to explore the 
      mechanism of abnormal implantation induced by CS2 from the perspective of immune 
      injury. METHODS: Mice were exposed to CS2 at different doses or at different time 
      points in the implantation phase to establish model 1 and model 2. For model 1, 
      mice were assigned to four groups to receive a single intraperitoneal injection 
      of low-dose CS2 (0.1 LD50, 157.8 mg/kg), middle-dose CS2 (0.2 LD50, 315.7 mg/kg), 
      and high-dose CS2 (0.4 LD50, 631.4 mg/kg) as well as an equal volume of olive oil 
      (control) on gestational day (GD) 4. For model 2, mice were assigned to four 
      groups to receive a single intraperitoneal injection of CS2 (0.4 LD50, 631.4 
      mg/kg) or an equal volume of olive oil (control) on GD3, GD4, GD5, and GD6. At 
      the end, single cell suspension of splenic lymphocytes was prepared. Cell 
      viability was measured by trypan blue staining, and the DNA damage of splenic 
      lymphocytes was evaluated by alkaline single cell gel electrophoresis assay. 
      RESULTS: The middle-dose and high-dose exposure groups showed significantly more 
      DNA damage of splenic lymphocytes than the control group (P < 0.01); there was 
      significant regression relationship between indicators of DNA damage and exposure 
      doses (P < 0.01). The GD3, GD4, GD5, and GD 6 exposure groups showed 
      significantly more DNA damage of splenic lymphocytes than the control group (P < 
      0.01), and the GD 4 exposure group had the most DNA damage. CONCLUSION: Exposure 
      to CS2 in the implantation phase can induce DNA damage of splenic lymphocytes in 
      pregnant mice, and the DNA damage was aggravated with the increase in CS2 
      concentration. GD4 may be the sensitive time point for DNA damage of splenic 
      lymphocytes induced by CS2 in pregnant mice.
FAU - Hu, Cheng-xia
AU  - Hu CX
AD  - Institute of Epidemiology and Health Statistics, School of Public Health, 
      Shandong University, Jinan 250012, China.
FAU - Zhang, Bing-zhen
AU  - Zhang BZ
FAU - Li, Chun-hui
AU  - Li CH
FAU - Wu, Yan-ling
AU  - Wu YL
FAU - Yang, Liu
AU  - Yang L
FAU - Wang, Zhi-ping
AU  - Wang ZP
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
JT  - Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng 
      zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases
JID - 8410840
RN  - S54S8B99E8 (Carbon Disulfide)
SB  - IM
MH  - Animals
MH  - Carbon Disulfide/*toxicity
MH  - DNA Damage/*drug effects
MH  - *Embryo Implantation
MH  - Female
MH  - Lymphocytes/*drug effects
MH  - Mice
MH  - Pregnancy
MH  - Spleen/cytology
EDAT- 2013/09/24 06:00
MHDA- 2014/11/14 06:00
CRDT- 2013/09/24 06:00
PHST- 2013/09/24 06:00 [entrez]
PHST- 2013/09/24 06:00 [pubmed]
PHST- 2014/11/14 06:00 [medline]
PST - ppublish
SO  - Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2013 Aug;31(8):576-80.