PMID- 24054499
OWN - NLM
STAT- MEDLINE
DCOM- 20140521
LR  - 20220310
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 34
IP  - 38
DP  - 2013 Dec
TI  - Umbilical cord and bone marrow mesenchymal stem cell seeding on macroporous 
      calcium phosphate for bone regeneration in rat cranial defects.
PG  - 9917-25
LID - S0142-9612(13)01073-9 [pii]
LID - 10.1016/j.biomaterials.2013.09.002 [doi]
AB  - Human umbilical cord mesenchymal stem cells (hUCMSCs) are inexhaustible and can 
      be harvested at a low cost without an invasive procedure. However, there has been 
      no report on comparing hUCMSCs with human bone marrow MSCs (hBMSCs) for bone 
      regeneration in vivo. The aim of this study was to investigate hUCMSC and hBMSC 
      seeding on macroporous calcium phosphate cement (CPC), and to compare their bone 
      regeneration in critical-sized cranial defects in rats. Cell attachment, 
      osteogenic differentiation and mineral synthesis on RGD-modified macroporous CPC 
      were investigated in vitro. Scaffolds with cells were implanted in 8-mm defects 
      of athymic rats. Bone regeneration was investigated via micro-CT and histological 
      analysis at 4, 12, and 24 weeks. Three groups were tested: CPC with hUCMSCs, CPC 
      with hBMSCs, and CPC control without cells. Percentage of live cells and cell 
      density on CPC in vitro were similarly good for hUCMSCs and hBMSCs. Both cells 
      had high osteogenic expressions of alkaline phosphatase, osteocalcin, collagen I, 
      and Runx2. Bone mineral density and trabecular thickness in hUCMSC and hBMSC 
      groups in vivo were greater than those of CPC control group. New bone amount for 
      hUCMSC-CPC and hBMSC-CPC constructs was increased by 57% and 88%, respectively, 
      while blood vessel density was increased by 15% and 20%, than CPC control group 
      at 24 weeks. hUCMSC-CPC and hBMSC-CPC groups generally had statistically similar 
      bone mineral density, new bone amount and vessel density. In conclusion, hUCMSCs 
      seeded on CPC were shown to match the bone regeneration efficacy of hBMSCs in 
      vivo for the first time. Both hUCMSC-CPC and hBMSC-CPC constructs generated much 
      more new bone and blood vessels than CPC without cells. Macroporous RGD-grafted 
      CPC with stem cell seeding is promising for craniofacial and orthopedic repairs.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Chen, Wenchuan
AU  - Chen W
AD  - State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, 
      Sichuan University, Chengdu, Sichuan 610041, China; Biomaterials & Tissue 
      Engineering Division, Department of Endodontics, Prosthodontics and Operative 
      Dentistry, University of Maryland Dental School, Baltimore, MD 21201, USA.
FAU - Liu, Jun
AU  - Liu J
FAU - Manuchehrabadi, Navid
AU  - Manuchehrabadi N
FAU - Weir, Michael D
AU  - Weir MD
FAU - Zhu, Zhimin
AU  - Zhu Z
FAU - Xu, Hockin H K
AU  - Xu HH
LA  - eng
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R21 DE022625/DE/NIDCR NIH HHS/United States
GR  - R01 DE14190/DE/NIDCR NIH HHS/United States
GR  - R21 DE22625/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130918
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Calcium Phosphates)
RN  - 0 (Collagen Type I)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 104982-03-8 (Osteocalcin)
RN  - 97Z1WI3NDX (calcium phosphate)
SB  - IM
MH  - Bone Marrow Cells/*cytology
MH  - Bone Regeneration/physiology
MH  - Calcium Phosphates/*chemistry
MH  - Cell Differentiation/physiology
MH  - Cells, Cultured
MH  - Collagen Type I/genetics/metabolism
MH  - Core Binding Factor Alpha 1 Subunit/genetics/metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology
MH  - Microscopy, Electron, Scanning
MH  - Osteocalcin/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - *Skull
MH  - Stem Cells/cytology
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/*cytology
MH  - X-Ray Microtomography
PMC - PMC4023544
MID - NIHMS523042
OTO - NOTNLM
OT  - Athymic rats
OT  - Bone regeneration
OT  - Calcium phosphate cement
OT  - Critical-sized cranial defect
OT  - RGD
OT  - Stem cells
EDAT- 2013/09/24 06:00
MHDA- 2014/05/23 06:00
PMCR- 2014/12/01
CRDT- 2013/09/24 06:00
PHST- 2013/08/12 00:00 [received]
PHST- 2013/09/02 00:00 [accepted]
PHST- 2013/09/24 06:00 [entrez]
PHST- 2013/09/24 06:00 [pubmed]
PHST- 2014/05/23 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - S0142-9612(13)01073-9 [pii]
AID - 10.1016/j.biomaterials.2013.09.002 [doi]
PST - ppublish
SO  - Biomaterials. 2013 Dec;34(38):9917-25. doi: 10.1016/j.biomaterials.2013.09.002. 
      Epub 2013 Sep 18.