PMID- 24055971
OWN - NLM
STAT- MEDLINE
DCOM- 20140829
LR  - 20131014
IS  - 1873-2976 (Electronic)
IS  - 0960-8524 (Linking)
VI  - 148
DP  - 2013 Nov
TI  - Production of biomass and lipid by the microalgae Chlorella protothecoides with 
      heterotrophic-Cu(II) stressed (HCuS) coupling cultivation.
PG  - 283-92
LID - S0960-8524(13)01411-9 [pii]
LID - 10.1016/j.biortech.2013.08.153 [doi]
AB  - This work for the first time investigated lipid accumulation by a two-stage 
      regime namely heterotrophic-Cu(II) stressed (HCuS) and underlying molecular basis 
      of lipid biosynthesis in Chlorella protothecoides cells. The results showed that 
      the optimized biomass and lipid yield were achieved by 6.47 g/L and 5.78 g/L with 
      this strategy. The fatty acids compositions (almost 100% of them are C15 to C20) 
      are ideal for preparing high quality biodiesel. Further, 30 differentially 
      expressed proteins response to HCuS were involved in carbohydrate metabolism, 
      carbon fixation, TCA cycle, lipid metabolism, protein biosynthesis, 
      transportation and regulation, ATP and RNA biosynthesis, nucleotide metabolism, 
      ROS scavenging. Especially, glycolysis pathway might be the important contributor 
      for lipid accumulation. In future, further functional analysis of these altered 
      proteins would help to reveal more concerning lipid biosynthesis pathway.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Li, Yuqin
AU  - Li Y
AD  - School of Chemical Engineering, Xiangtan University, Xiangtan, PR China. 
      Electronic address: yuqinli2004@aliyun.com.
FAU - Mu, Jinxiu
AU  - Mu J
FAU - Chen, Di
AU  - Chen D
FAU - Han, Fangxin
AU  - Han F
FAU - Xu, Hua
AU  - Xu H
FAU - Kong, Feng
AU  - Kong F
FAU - Xie, Feng
AU  - Xie F
FAU - Feng, Bo
AU  - Feng B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130905
PL  - England
TA  - Bioresour Technol
JT  - Bioresource technology
JID - 9889523
RN  - 0 (Algal Proteins)
RN  - 0 (Lipids)
RN  - 789U1901C5 (Copper)
SB  - IM
MH  - Algal Proteins/metabolism
MH  - *Biomass
MH  - Cell Culture Techniques/*methods
MH  - Chlorella/cytology/drug effects/*metabolism
MH  - Copper/*toxicity
MH  - Electrophoresis, Gel, Two-Dimensional
MH  - Gene Ontology
MH  - Heterotrophic Processes/*drug effects
MH  - Lipids/*biosynthesis
MH  - Metabolic Networks and Pathways/drug effects
MH  - Microalgae/cytology/drug effects/*metabolism
MH  - Models, Biological
MH  - Proteomics
MH  - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
MH  - Stress, Physiological/drug effects
OTO - NOTNLM
OT  - Biomass
OT  - Chlorella protothecoides
OT  - Heterotrophic-Cu(II) stressed
OT  - Lipid
OT  - Proteomics
EDAT- 2013/09/24 06:00
MHDA- 2014/08/30 06:00
CRDT- 2013/09/24 06:00
PHST- 2013/07/02 00:00 [received]
PHST- 2013/08/24 00:00 [revised]
PHST- 2013/08/27 00:00 [accepted]
PHST- 2013/09/24 06:00 [entrez]
PHST- 2013/09/24 06:00 [pubmed]
PHST- 2014/08/30 06:00 [medline]
AID - S0960-8524(13)01411-9 [pii]
AID - 10.1016/j.biortech.2013.08.153 [doi]
PST - ppublish
SO  - Bioresour Technol. 2013 Nov;148:283-92. doi: 10.1016/j.biortech.2013.08.153. Epub 
      2013 Sep 5.