PMID- 24057226
OWN - NLM
STAT- MEDLINE
DCOM- 20141010
LR  - 20131016
IS  - 1474-9092 (Electronic)
IS  - 1474-905X (Linking)
VI  - 12
IP  - 11
DP  - 2013 Nov
TI  - Rapid differentiation and quantification of live/dead cancer cells using 
      differential photochemical behavior of acridine orange.
PG  - 1921-6
LID - 10.1039/c3pp50165e [doi]
AB  - This paper demonstrates for the first time a simple analytical method for 
      differentiation and quantification of dead/live cancer cells using acridine 
      orange (AO) enabled fluorescence spectroscopic techniques. Based on the 
      differential fluorescence (live cells fluoresce green and dead cells orange) 
      exhibited when intercalated with AO, the live/dead cells can be easily 
      differentiated. The optimal AO concentration for enhanced sensitive 
      differentiation has been optimized as 0.001%. These studies offer a promising 
      application for rapid differentiation and quantification of live/dead cells in 
      the case of cytotoxic treatment/therapies within several minutes.
FAU - Manikandan, M
AU  - Manikandan M
AD  - Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai 
      Road, 80424, Taiwan.
FAU - Wu, Hui-Fen
AU  - Wu HF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Photochem Photobiol Sci
JT  - Photochemical & photobiological sciences : Official journal of the European 
      Photochemistry Association and the European Society for Photobiology
JID - 101124451
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/*chemistry/*radiation effects
MH  - Animals
MH  - Cell Death
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Mice
MH  - Neuroblastoma/*pathology
MH  - Photochemical Processes
MH  - Spectrometry, Fluorescence
MH  - Tumor Cells, Cultured
EDAT- 2013/09/24 06:00
MHDA- 2014/10/11 06:00
CRDT- 2013/09/24 06:00
PHST- 2013/09/24 06:00 [entrez]
PHST- 2013/09/24 06:00 [pubmed]
PHST- 2014/10/11 06:00 [medline]
AID - 10.1039/c3pp50165e [doi]
PST - ppublish
SO  - Photochem Photobiol Sci. 2013 Nov;12(11):1921-6. doi: 10.1039/c3pp50165e.