PMID- 24058147
OWN - NLM
STAT- MEDLINE
DCOM- 20140923
LR  - 20181202
IS  - 1471-9053 (Electronic)
IS  - 0032-0781 (Linking)
VI  - 54
IP  - 11
DP  - 2013 Nov
TI  - Two DYW subclass PPR proteins are involved in RNA editing of ccmFc and atp9 
      transcripts in the moss Physcomitrella patens: first complete set of PPR editing 
      factors in plant mitochondria.
PG  - 1907-16
LID - 10.1093/pcp/pct132 [doi]
AB  - The moss Physcomitrella patens has 11 RNA editing sites in mitochondrial 
      transcripts. We previously identified six DYW subclass pentatricopeptide repeat 
      (PPR) proteins as RNA editing factors for nine out of 11 sites. In this study, we 
      identified two novel DYW subclass PPR proteins, PpPPR_65 and PpPPR_98, as RNA 
      editing factors. Disruption of the PpPPR_65 gene resulted in a complete loss of 
      RNA editing at two neighboring sites, ccmFc-C103 and ccmFc-C122, in the 
      mitochondrial ccmFc transcript. To confirm this result, we further generated 
      PpPPR_65 knockdown (KD) mutants by an inducible RNA interference (RNAi) system. 
      The generated RNAi lines displayed reduced levels of RNA editing at both 
      ccmFc-C103 and ccmFc-C122 sites. Next, we characterized the function of PpPPR_98 
      by constructing a KD mutant of PpPPR_98 expression. The KD mutant showed a 30% 
      reduction in the level of atp9-C92 editing. When PpPPR_98 cDNA was introduced 
      into the KD mutant, RNA editing levels were restored to the wild-type level. This 
      indicates that PpPPR_98 is an editing factor for the atp9-C92 site. The 
      recombinant PpPPR_98 protein bound to the upstream sequence of the editing site 
      that was created by splicing of atp9 transcript. This suggests that atp9 RNA 
      editing occurs after splicing of atp9 transcript. Our present and previous data 
      provide the first evidence that all 11 known editing events require at least 
      eight DYW subclass PPR proteins in the moss mitochondria.
FAU - Ichinose, Mizuho
AU  - Ichinose M
AD  - Center for Gene Research, Nagoya University, Chikusa-ku, Nagoya, 464-8602 Japan.
FAU - Sugita, Chieko
AU  - Sugita C
FAU - Yagi, Yusuke
AU  - Yagi Y
FAU - Nakamura, Takahiro
AU  - Nakamura T
FAU - Sugita, Mamoru
AU  - Sugita M
LA  - eng
SI  - GENBANK/AB830083
SI  - GENBANK/AB830784
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130920
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 0 (DNA, Complementary)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Plant Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Mitochondrial)
RN  - 0 (RNA, Plant)
RN  - 0 (Recombinant Proteins)
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Base Sequence
MH  - Bryopsida/*genetics/growth & development
MH  - DNA, Complementary/genetics
MH  - Gene Knockout Techniques
MH  - Mitochondria/genetics
MH  - Mitochondrial Proteins/genetics
MH  - Molecular Sequence Data
MH  - Mutation
MH  - Plant Proteins/*genetics
MH  - Protein Binding
MH  - RNA/genetics
MH  - RNA Editing/*genetics
MH  - RNA Interference
MH  - RNA Splicing
MH  - RNA, Messenger/genetics
MH  - RNA, Mitochondrial
MH  - RNA, Plant/genetics
MH  - Recombinant Proteins
MH  - Sequence Analysis, DNA
OTO - NOTNLM
OT  - Mitochondria
OT  - Moss
OT  - PPR protein
OT  - Physcomitrella patens
OT  - RNA editing
EDAT- 2013/09/24 06:00
MHDA- 2014/09/24 06:00
CRDT- 2013/09/24 06:00
PHST- 2013/09/24 06:00 [entrez]
PHST- 2013/09/24 06:00 [pubmed]
PHST- 2014/09/24 06:00 [medline]
AID - pct132 [pii]
AID - 10.1093/pcp/pct132 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2013 Nov;54(11):1907-16. doi: 10.1093/pcp/pct132. Epub 2013 
      Sep 20.