PMID- 24060212
OWN - NLM
STAT- MEDLINE
DCOM- 20150714
LR  - 20161021
IS  - 0219-3108 (Electronic)
IS  - 1015-9584 (Linking)
VI  - 37
IP  - 2
DP  - 2014 Apr
TI  - Upregulation of the proinflammatory cytokine-induced neutrophil chemoattractant-1 
      and monocyte chemoattractant protein-1 in rats' intestinal anastomotic wound 
      healing--does it matter?
PG  - 86-92
LID - S1015-9584(13)00073-0 [pii]
LID - 10.1016/j.asjsur.2013.07.016 [doi]
AB  - BACKGROUND: The proinflammatory cytokines and growth-promoting factor are 
      essential components of the wound healing process. We hypothesized that under 
      healthy conditions, faster healing of intestinal anastomotic wound is due to an 
      early upregulation of proinflammatory cytokines, cytokine-induced neutrophil 
      chemoattractant-1 (CINC-1) that is followed by a quicker upregulation of 
      homeostatic chemokine, monocyte chemoattractant protein-1 (MCP-1) and late 
      upregulation of transforming growth factor (TGF-beta). METHODS: We characterized the 
      time course of CINC-1, MCP-1 and TGF-beta release at four wounds (skin, muscle, 
      small bowel, and colonic anastomosis) after surgery on 38 juvenile male Sprague 
      Dawley rats. The tissue samples of each site were harvested at 0 (control), 1, 3, 
      5, 7 and 14 days postoperatively (n = 6-8/group) and analyzed by ELISA kits for 
      CINC-1, MCP-1 and TGF-beta. RESULTS: CINC-1 expression peaked earlier in muscle and 
      colonic wounds when compared to skin and small bowel. MCP-1 levels were elevated 
      early in skin and muscle wounds, but later expression of MCP-1 was shown in 
      colonic wounds. TGF-beta levels were unchanged in all wound sites. CONCLUSION: An 
      earlier peak in CINC-1 levels and later expression of MCP-1 were seen in colonic 
      wounds, but no significant increase in TGF-beta levels was observed. These findings 
      support the early healing process in intestinal anastomotic wounds.
CI  - Copyright (c) 2013. Published by Elsevier B.V.
FAU - Alzoghaibi, Mohammed A
AU  - Alzoghaibi MA
AD  - Center of Excellence in Biotechnology Research, King Saud University, Riyadh, 
      Saudi Arabia; Department of Physiology, College of Medicine, King Saud 
      University, Riyadh, Saudi Arabia. Electronic address: zzoghaibi@gmail.com.
FAU - Zubaidi, Ahmed M
AU  - Zubaidi AM
AD  - Department of Surgery, College of Medicine, King Saud University, Riyadh, Saudi 
      Arabia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130921
PL  - Netherlands
TA  - Asian J Surg
JT  - Asian journal of surgery
JID - 8900600
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-8)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Chemokine CCL2/*physiology
MH  - Colon/injuries
MH  - Interleukin-8/*physiology
MH  - Male
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Transforming Growth Factor beta/physiology
MH  - *Up-Regulation
MH  - Wound Healing/*physiology
OTO - NOTNLM
OT  - CINC-1
OT  - MCP-1
OT  - TGF-beta
OT  - intestinal anastomosis
OT  - wound
EDAT- 2013/09/26 06:00
MHDA- 2015/07/15 06:00
CRDT- 2013/09/25 06:00
PHST- 2013/01/22 00:00 [received]
PHST- 2013/07/13 00:00 [revised]
PHST- 2013/07/23 00:00 [accepted]
PHST- 2013/09/25 06:00 [entrez]
PHST- 2013/09/26 06:00 [pubmed]
PHST- 2015/07/15 06:00 [medline]
AID - S1015-9584(13)00073-0 [pii]
AID - 10.1016/j.asjsur.2013.07.016 [doi]
PST - ppublish
SO  - Asian J Surg. 2014 Apr;37(2):86-92. doi: 10.1016/j.asjsur.2013.07.016. Epub 2013 
      Sep 21.