PMID- 24060556
OWN - NLM
STAT- MEDLINE
DCOM- 20140623
LR  - 20161125
IS  - 1217-8950 (Print)
IS  - 1217-8950 (Linking)
VI  - 60
IP  - 3
DP  - 2013 Sep
TI  - Spatial and temporal changes of bacterial communities inhabiting the well waters 
      of Harkany Spa.
PG  - 329-43
LID - 10.1556/AMicr.60.2013.3.8 [doi]
AB  - In this study, changes in the bacterial community composition of the well waters 
      of Harkany Spa were examined. Physical and chemical properties of mixing 
      subsurface cold and thermal karst waters were correlated to shifts in bacterial 
      community structures analyzed by denaturing gradient gel electrophoresis (DGGE) 
      and principal component analysis (PCA). In addition, mineral components of the 
      pellets were investigated by scanning electron microscopy. Samples from the 
      effluent waters of Budostapolca I and II, Matty and Thermal VI wells were taken 
      seasonally in 2007 and 2008. The comparison of the results of DGGE and PCA 
      analyses showed that bacterial communities from the Budostapolca wells were 
      distinct from those of Matty and Thermal VI, but seasonal changes were not 
      detected. According to the phylogenetic analysis of the excised DGGE bands, 
      presence of chemolithotrophic Proteobacteria (Thiobacillus, Thiothrix, and 
      distant relatives of Sulfurospirillum) were typical in the Budostapolca wells, 
      while members of Actinobacteria (Plantibacter, Actinobacterium, Microbacterium) 
      and Firmicutes (Planococcus) were characteristic to the Matty and Thermal VI 
      wells. In the pellets pyrite framboid crystals were observed by electron 
      microscopy, which are minerals known to be biologically induced by dissimilatory 
      iron- and sulfur (sulfate)-reducing bacteria.
FAU - Miseta, Roland
AU  - Miseta R
AD  - Eotvos Lorand University Department of Microbiology Pazmany P. setany 1/C H-1117 
      Budapest Hungary.
FAU - Palatinszky, Marton
AU  - Palatinszky M
FAU - Makk, Judit
AU  - Makk J
FAU - Marialigeti, Karoly
AU  - Marialigeti K
FAU - Borsodi, Andrea K
AU  - Borsodi AK
LA  - eng
SI  - GENBANK/FR851863
SI  - GENBANK/FR851864
SI  - GENBANK/FR851865
SI  - GENBANK/FR851866
SI  - GENBANK/FR851867
SI  - GENBANK/FR851868
SI  - GENBANK/FR851869
SI  - GENBANK/FR851870
SI  - GENBANK/FR851871
SI  - GENBANK/FR851872
SI  - GENBANK/FR851873
SI  - GENBANK/FR851874
SI  - GENBANK/FR851875
SI  - GENBANK/FR851876
PT  - Journal Article
PL  - Hungary
TA  - Acta Microbiol Immunol Hung
JT  - Acta microbiologica et immunologica Hungarica
JID - 9434021
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sulfides)
RN  - 132N09W4PR (pyrite)
RN  - E1UOL152H7 (Iron)
SB  - IM
MH  - Actinobacteria/*classification/genetics/growth & development/isolation & 
      purification
MH  - Base Sequence
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - Denaturing Gradient Gel Electrophoresis
MH  - Gram-Positive Bacteria/*classification/genetics/growth & development/isolation & 
      purification
MH  - Hungary
MH  - Iron/chemistry
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Principal Component Analysis
MH  - Proteobacteria/*classification/genetics/growth & development/isolation & 
      purification
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sulfides/analysis/chemistry
MH  - *Water Microbiology
MH  - Water Wells/chemistry/*microbiology
EDAT- 2013/09/26 06:00
MHDA- 2014/06/24 06:00
CRDT- 2013/09/25 06:00
PHST- 2013/09/25 06:00 [entrez]
PHST- 2013/09/26 06:00 [pubmed]
PHST- 2014/06/24 06:00 [medline]
AID - J07H5871P117V671 [pii]
AID - 10.1556/AMicr.60.2013.3.8 [doi]
PST - ppublish
SO  - Acta Microbiol Immunol Hung. 2013 Sep;60(3):329-43. doi: 
      10.1556/AMicr.60.2013.3.8.