PMID- 24063161
OWN - NLM
STAT- MEDLINE
DCOM- 20140724
LR  - 20181202
IS  - 1002-1892 (Print)
IS  - 1002-1892 (Linking)
VI  - 27
IP  - 7
DP  - 2013 Jul
TI  - [Effect of serum from severe burn patients on biology characteristics of human 
      umbilical cord mesenchymal stem cells].
PG  - 769-74
AB  - OBJECTIVE: To investigate the effect of the serum from severe burn patients on 
      the biology characteristics of human umbilical cord mesenchymal stem cells 
      (hUCMSCs) in vitro, so as to explore the feasibility of hUCMSCs transplantation 
      for treating severe burn. METHODS: The 3rd passage of hUCMSCs were randomly 
      divided into 3 groups: 10% fetal bovine serum group (group A), 10% normal serum 
      group (group B), and 10% burn serum group (group C). At 24 hours, 72 hours, and 6 
      days after culture, the cell morphology and density were observed by inverted 
      microscope; the cell proliferation was assessed by MTT; after 6 days of culture, 
      the cell cycle by propidium iodide staining and flow cytometry, the apoptosis by 
      acridine orange/ ethidium bromide staining, and the cell senescence by 
      beta-galactosidase staining; the levels of tumor necrosis factor alpha 
      (TNF-alpha), interleukin 1 (IL-1), platelet-derived growth factor (PDGF), and 
      insulin-like growth factor 1 (IGF-1) in serum were detected by a double-antibody 
      sandwich ELISA kit. RESULTS: hUCMSCs were long spindle/polygon in 3 groups. The 
      cell fusion of group C was obviously faster than that in group A and group B. The 
      cell proliferation curves showed that the velocity and number of cell 
      proliferation in group C were significantly higher than those in group A and 
      group B at 2-6 days after culture (P < 0.05). The rates of proliferation period 
      (S) of hUCMSCs were 9.21% +/- 1.02%, 11.79% +/- 1.87%, and 20.54% +/- 2.03%, 
      respectively in groups A, B, and C at 6 days, and group C was significantly 
      higher than that of group A and group B (P < 0.05). The hUCMSCs showed normal 
      morphology and structure in 3 groups, and no apoptosis cells was observed. The 
      positive cells percentage of group C (2.6% +/- 0.1%) was significantly lower than 
      that of group A (4.8% +/- 0.2%) and group B (3.8% +/- 0.4%) (P < 0.05). The 
      levels of TNF-alpha, IL-1, PDGF, and IGF-1 in group C were significantly higher 
      than those in group B (P < 0.05). CONCLUSION: The higher levels of cytokines in 
      serum from the severe burn patients can significantly stimulate hUCMSCs 
      proliferation, prevent cells apoptosis, and reduce cells senescence. Therefore, 
      it is feasible to use hUCMSCs transplantation for treating severe burn patients.
FAU - Liu, Lingying
AU  - Liu L
AD  - Department of Burn and Plastic Surgery, First Hospital Affiliated to General 
      Hospital of Chinese PLA, Beijing, 100048, P.R. China.
FAU - Chai, Jiake
AU  - Chai J
FAU - Hou, Yusen
AU  - Hou Y
FAU - Duan, Hongjie
AU  - Duan H
FAU - Yu, Yonghui
AU  - Yu Y
FAU - Yin, Huinan
AU  - Yin H
FAU - Hu, Quan
AU  - Hu Q
FAU - Fan, Jun
AU  - Fan J
FAU - Du, Jundong
AU  - Du J
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi
JT  - Zhongguo xiu fu chong jian wai ke za zhi = Zhongguo xiufu chongjian waike zazhi = 
      Chinese journal of reparative and reconstructive surgery
JID - 9425194
RN  - 0 (Culture Media)
RN  - 0 (Cytokines)
RN  - 0 (Platelet-Derived Growth Factor)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Adult
MH  - Apoptosis
MH  - Burns/*blood/metabolism
MH  - Cell Culture Techniques/methods
MH  - *Cell Proliferation
MH  - Cells, Cultured
MH  - Culture Media/chemistry
MH  - Cytokines/*blood
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Flow Cytometry
MH  - Humans
MH  - Mesenchymal Stem Cells/*cytology/metabolism/ultrastructure
MH  - Platelet-Derived Growth Factor/analysis
MH  - Tumor Necrosis Factor-alpha/blood
MH  - Umbilical Cord/*cytology
MH  - Young Adult
EDAT- 2013/09/26 06:00
MHDA- 2014/07/25 06:00
CRDT- 2013/09/26 06:00
PHST- 2013/09/26 06:00 [entrez]
PHST- 2013/09/26 06:00 [pubmed]
PHST- 2014/07/25 06:00 [medline]
PST - ppublish
SO  - Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2013 Jul;27(7):769-74.