PMID- 24063947
OWN - NLM
STAT- MEDLINE
DCOM- 20140611
LR  - 20211021
IS  - 1873-2763 (Electronic)
IS  - 8756-3282 (Print)
IS  - 1873-2763 (Linking)
VI  - 57
IP  - 2
DP  - 2013 Dec
TI  - The plastic nature of the human bone-periodontal ligament-tooth fibrous joint.
PG  - 455-67
LID - S8756-3282(13)00359-1 [pii]
LID - 10.1016/j.bone.2013.09.007 [doi]
AB  - This study investigates bony protrusions within a narrowed periodontal ligament 
      space (PDL-space) of a human bone-PDL-tooth fibrous joint by mapping structural, 
      biochemical, and mechanical heterogeneity. Higher resolution structural 
      characterization was achieved via complementary atomic force microscopy (AFM), 
      nano-transmission X-ray microscopy (nano-TXM), and microtomography (MicroXCT). 
      Structural heterogeneity was correlated to biochemical and elemental composition, 
      illustrated via histochemistry and microprobe X-ray fluorescence analysis 
      (mu-XRF), and mechanical heterogeneity evaluated by AFM-based nanoindentation. 
      Results demonstrated that the narrowed PDL-space was due to invasion of bundle 
      bone (BB) into PDL-space. Protruded BB had a wider range with higher elastic 
      modulus values (2-8GPa) compared to lamellar bone (0.8-6GPa), and increased 
      quantities of Ca, P and Zn as revealed by mu-XRF. Interestingly, the hygroscopic 
      10-30mum interface between protruded BB and lamellar bone exhibited higher X-ray 
      attenuation similar to cement lines and lamellae within bone. Localization of the 
      small leucine rich proteoglycan biglycan (BGN) responsible for mineralization was 
      observed at the PDL-bone interface and around the osteocyte lacunae. Based on 
      these results, it can be argued that the LB-BB interface was the original site of 
      PDL attachment, and that the genesis of protruded BB identified as protrusions 
      occurred as a result of shift in strain. We emphasize the importance of bony 
      protrusions within the context of organ function and that additional study is 
      warranted.
CI  - (c) 2013. Published by Elsevier Inc. All rights reserved.
FAU - Ho, Sunita P
AU  - Ho SP
AD  - Division of Biomaterials and Bioengineering, Department of Preventive and 
      Restorative Dental Sciences, University of California San Francisco, San 
      Francisco, CA, USA. Electronic address: sunita.ho@ucsf.edu.
FAU - Kurylo, Michael P
AU  - Kurylo MP
FAU - Grandfield, Kathryn
AU  - Grandfield K
FAU - Hurng, Jonathan
AU  - Hurng J
FAU - Herber, Ralf-Peter
AU  - Herber RP
FAU - Ryder, Mark I
AU  - Ryder MI
FAU - Altoe, Virginia
AU  - Altoe V
FAU - Aloni, Shaul
AU  - Aloni S
FAU - Feng, Jian Q
AU  - Feng JQ
FAU - Webb, Samuel
AU  - Webb S
FAU - Marshall, Grayson W
AU  - Marshall GW
FAU - Curtis, Donald
AU  - Curtis D
FAU - Andrews, Joy C
AU  - Andrews JC
FAU - Pianetta, Piero
AU  - Pianetta P
LA  - eng
GR  - R00 DE018212/DE/NIDCR NIH HHS/United States
GR  - R00DE018212/DE/NIDCR NIH HHS/United States
GR  - T32 DE007306/DE/NIDCR NIH HHS/United States
GR  - S10RR026645/RR/NCRR NIH HHS/United States
GR  - R01 DE022032/DE/NIDCR NIH HHS/United States
GR  - S10 RR026645/RR/NCRR NIH HHS/United States
GR  - T32 DE07306/DE/NIDCR NIH HHS/United States
GR  - K99 DE018212/DE/NIDCR NIH HHS/United States
GR  - R01DE022032/DE/NIDCR NIH HHS/United States
GR  - R01 EB004321/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130921
PL  - United States
TA  - Bone
JT  - Bone
JID - 8504048
RN  - 0 (Biglycan)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (FMOD protein, human)
RN  - 0 (Proteoglycans)
RN  - 126468-95-9 (Fibromodulin)
RN  - 27YLU75U4W (Phosphorus)
RN  - J41CSQ7QDS (Zinc)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Biglycan/metabolism
MH  - Biomechanical Phenomena
MH  - Bone and Bones/diagnostic imaging/*physiology/ultrastructure
MH  - Calcium/metabolism
MH  - Dental Cementum/diagnostic imaging/physiology
MH  - Elastic Modulus
MH  - Extracellular Matrix Proteins/metabolism
MH  - Fibromodulin
MH  - Fluorescence
MH  - Humans
MH  - Immunohistochemistry
MH  - Joints/*physiology/ultrastructure
MH  - Microscopy, Atomic Force
MH  - Models, Biological
MH  - Periodontal Ligament/cytology/diagnostic imaging/*physiology/ultrastructure
MH  - Phosphorus/metabolism
MH  - Proteoglycans/metabolism
MH  - Surface Properties
MH  - Tooth/diagnostic imaging/*physiology/ultrastructure
MH  - X-Ray Microtomography
MH  - Zinc/metabolism
PMC - PMC3938967
MID - NIHMS526660
OTO - NOTNLM
OT  - Alveolar bone
OT  - Bone functional adaptation
OT  - Bone-PDL-tooth fibrous joint
OT  - Bundle bone
OT  - Discontinuities
OT  - Periodontal ligament
EDAT- 2013/09/26 06:00
MHDA- 2014/06/12 06:00
PMCR- 2014/12/01
CRDT- 2013/09/26 06:00
PHST- 2013/05/13 00:00 [received]
PHST- 2013/09/05 00:00 [revised]
PHST- 2013/09/11 00:00 [accepted]
PHST- 2013/09/26 06:00 [entrez]
PHST- 2013/09/26 06:00 [pubmed]
PHST- 2014/06/12 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - S8756-3282(13)00359-1 [pii]
AID - 10.1016/j.bone.2013.09.007 [doi]
PST - ppublish
SO  - Bone. 2013 Dec;57(2):455-67. doi: 10.1016/j.bone.2013.09.007. Epub 2013 Sep 21.