PMID- 24064350
OWN - NLM
STAT- MEDLINE
DCOM- 20131213
LR  - 20200204
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 440
IP  - 2
DP  - 2013 Oct 18
TI  - The cAMP response element binding protein (CREB) is activated by insulin-like 
      growth factor-1 (IGF-1) and regulates myostatin gene expression in skeletal 
      myoblast.
PG  - 258-64
LID - S0006-291X(13)01548-9 [pii]
LID - 10.1016/j.bbrc.2013.09.067 [doi]
AB  - Myostatin, a member of the Transforming Growth Factor beta (TGF-beta) superfamily, 
      plays an important role as a negative regulator of skeletal muscle growth and 
      differentiation. We have previously reported that IGF-1 induces a transient 
      myostatin mRNA expression, through the activation of the Nuclear Factor of 
      Activated T cells (NFAT) in an IP3/calcium-dependent manner. Here we examined the 
      activation of CREB transcription factor as downstream targets of IGF-1 during 
      myoblast differentiation and its role as a regulator of myostatin gene 
      expression. In cultured skeletal myoblast, IGF-1 induced the phosphorylation and 
      transcriptional activation of CREB via IGF-1 Receptor/Phosphatidylinositol 
      3-Kinase (PI3K)/Phospholipase C gamma (PLC gamma), signaling pathways. Also, IGF-1 
      induced calcium-dependent molecules such as Calmodulin Kinase II (CaMK II), 
      Extracellular signal-regulated Kinases (ERK), Protein Kinase C (PKC). 
      Additionally, we examined myostatin mRNA levels and myostatin promoter activity 
      in differentiated myoblasts stimulated with IGF-1. We found a significant 
      increase in mRNA contents of myostatin and its reporter activity after treatment 
      with IGF-1. The expression of myostatin in differentiated myoblast was 
      downregulated by the transfection of siRNA-CREB and by pharmacological inhibitors 
      of the signaling pathways involved in CREB activation. By using pharmacological 
      and genetic approaches together these data demonstrate that IGF-1 regulates the 
      myostatin gene expression via CREB transcription factor during muscle cell 
      differentiation.
CI  - Copyright (c) 2013 Elsevier Inc. All rights reserved.
FAU - Zuloaga, R
AU  - Zuloaga R
AD  - Facultad de Ciencias Biologicas, Universidad Andres Bello, Santiago, Chile.
FAU - Fuentes, E N
AU  - Fuentes EN
FAU - Molina, A
AU  - Molina A
FAU - Valdes, J A
AU  - Valdes JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130921
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Benzylamines)
RN  - 0 (Chromones)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Morpholines)
RN  - 0 (Mstn protein, rat)
RN  - 0 (Myostatin)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Sulfonamides)
RN  - 139298-40-1 (KN 93)
RN  - 31M2U1DVID (2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - DH2M523P0H (Genistein)
RN  - EC 2.7.10.1 (Receptor, IGF Type 1)
RN  - EC 2.7.11.17 (Calcium-Calmodulin-Dependent Protein Kinase Type 2)
RN  - EC 3.1.4.3 (Phospholipase C gamma)
SB  - IM
MH  - Animals
MH  - Benzylamines/pharmacology
MH  - Calcium-Calmodulin-Dependent Protein Kinase Type 2/antagonists & 
      inhibitors/physiology
MH  - Cell Differentiation/physiology
MH  - Chromones/pharmacology
MH  - Cyclic AMP Response Element-Binding Protein/*metabolism
MH  - Gene Expression Regulation
MH  - Genistein/pharmacology
MH  - Insulin-Like Growth Factor I/*pharmacology
MH  - Morpholines/pharmacology
MH  - Myoblasts, Skeletal/*metabolism
MH  - Myostatin/*biosynthesis/genetics
MH  - Phosphatidylinositol 3-Kinases/physiology
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Phospholipase C gamma/physiology
MH  - Phosphorylation
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Receptor, IGF Type 1/antagonists & inhibitors/physiology
MH  - Signal Transduction/physiology
MH  - Sulfonamides/pharmacology
OTO - NOTNLM
OT  - CREB
OT  - Calcium signaling
OT  - IGF-1
OT  - IP(3)
OT  - Myostatin
EDAT- 2013/09/26 06:00
MHDA- 2013/12/18 06:00
CRDT- 2013/09/26 06:00
PHST- 2013/09/03 00:00 [received]
PHST- 2013/09/12 00:00 [accepted]
PHST- 2013/09/26 06:00 [entrez]
PHST- 2013/09/26 06:00 [pubmed]
PHST- 2013/12/18 06:00 [medline]
AID - S0006-291X(13)01548-9 [pii]
AID - 10.1016/j.bbrc.2013.09.067 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2013 Oct 18;440(2):258-64. doi: 
      10.1016/j.bbrc.2013.09.067. Epub 2013 Sep 21.