PMID- 24064679 OWN - NLM STAT- MEDLINE DCOM- 20140513 LR - 20180816 IS - 1791-2431 (Electronic) IS - 1021-335X (Linking) VI - 30 IP - 6 DP - 2013 Dec TI - Mature BDNF promotes the growth of glioma cells in vitro. PG - 2719-24 LID - 10.3892/or.2013.2746 [doi] AB - High-grade glioma is incurable and is associated with a short survival time and a poor prognosis. There are two forms of brain-derived neurotrophic factor (BDNF), proBDNF and mature BDNF, which exert opposite effects. Their diverse actions are mediated through two different transmembrane receptor signalling systems: p75NTR and TrkB. The important roles of the BDNF/TrkB signalling system in tumour cell proliferation and survival have been demonstrated. However, few studies have been able to distinguish mature BDNF from proBDNF due to the limitation of specific antibodies. Using specific proBDNF antibodies, we demonstrated that the proBDNF/p75NTR pathway appears to inhibit malignant glioma cell growth and migration. In the present study using specific mature BDNF antibodies, we found that mature BDNF inhibited C6 glioma cell apoptosis and increased cell growth and migration in vitro. Our data suggest that the counterbalance between mature BDNF and proBDNF may regulate tumour growth. FAU - Xiong, Jing AU - Xiong J AD - Key Laboratory of Stem Cells and Regenerative Medicine, Institute of Molecular and Clinical Medicine, Kunming Medical University, Kunming, Yunnan, P.R. China. FAU - Zhou, Li AU - Zhou L FAU - Lim, Yoon AU - Lim Y FAU - Yang, Miao AU - Yang M FAU - Zhu, Yu-Hong AU - Zhu YH FAU - Li, Zhi-Wei AU - Li ZW FAU - Zhou, Fiona H AU - Zhou FH FAU - Xiao, Zhi-Cheng AU - Xiao ZC FAU - Zhou, Xin-Fu AU - Zhou XF LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20130920 PL - Greece TA - Oncol Rep JT - Oncology reports JID - 9422756 RN - 0 (Antibodies) RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (NGFR protein, human) RN - 0 (Nerve Tissue Proteins) RN - 0 (Receptors, Nerve Growth Factor) RN - 7171WSG8A2 (BDNF protein, human) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Antibodies/pharmacology MH - Apoptosis/*genetics MH - Brain-Derived Neurotrophic Factor/*genetics/metabolism MH - Cell Line, Tumor MH - Cell Movement/genetics MH - Cell Proliferation/drug effects MH - Glioma/*genetics/metabolism/pathology MH - Humans MH - Neoplasm Grading MH - Nerve Tissue Proteins/genetics/metabolism MH - Neurons/metabolism MH - Prognosis MH - Receptor, trkB/genetics/metabolism MH - Receptors, Nerve Growth Factor/genetics/metabolism MH - *Signal Transduction EDAT- 2013/09/26 06:00 MHDA- 2014/05/14 06:00 CRDT- 2013/09/26 06:00 PHST- 2013/07/24 00:00 [received] PHST- 2013/08/26 00:00 [accepted] PHST- 2013/09/26 06:00 [entrez] PHST- 2013/09/26 06:00 [pubmed] PHST- 2014/05/14 06:00 [medline] AID - 10.3892/or.2013.2746 [doi] PST - ppublish SO - Oncol Rep. 2013 Dec;30(6):2719-24. doi: 10.3892/or.2013.2746. Epub 2013 Sep 20.