PMID- 24065119
OWN - NLM
STAT- MEDLINE
DCOM- 20141113
LR  - 20131002
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Linking)
VI  - 8
IP  - 5
DP  - 2013 Nov
TI  - Effects of shuangtengbitong tincture on collagen‑induced arthritis in rats.
PG  - 1479-85
LID - 10.3892/mmr.2013.1693 [doi]
AB  - Shuangtengbitong tincture (STBT), a clinical prescription from the Fujian 
      University of Traditional Chinese Medicine (TCM) Affiliated People's Hospital 
      (Fuzhou, China), has been used in the treatment of rheumatoid arthritis (RA) for 
      ~10 years. The aim of the current study was to con fi rm the anti-RA effect of STBT 
      and to evaluate the potential mechanisms underlying collagen-induced arthritis 
      (CIA) in rats. CIA model Wistar rats were induced with bovine type II collagen. 
      The rats were immunized with CIA and were treated with STBT (0.5 and 2 
      ml/injection) and votalin (~1 cm/injection) continuously for ~1 month. Following 
      treatment, the pathological sections of CIA rat joints were observed by 
      hematoxylin and eosin staining, expression of toll-like receptor-4 (TLR4), 
      myeloid differentiation factor 88 (MyD88) and nuclear transcription factor-kappaB 
      (NF-kappaB) were investigated by western blot analysis and reverse 
      transcription-polymerase chain reaction (RT-PCR) analysis. Following treatment, 
      STBT significantly suppressed paw swelling (P<0.05) compared with the model group 
      and increased body weight. STBT also reversed pathological changes, STBT‑treated 
      rats showed a signi fi cant improvement of synovial hyperplasia, in fl ammatory 
      in fi ltration, and cartilage and bone destruction. The levels of protein and mRNA 
      expression of TLR4, MyD88 and NF-kappaB were markedly suppressed in the synovial 
      tissue of STBT‑ and votalin-treated rats. In addition, STBT showed marked 
      inhibition of the levels of protein and mRNA expression of TLR4, MyD88 and NF-kappaB 
      at an STBT volume ranging between 1 and 4 ml/day, indicating that the inhibition 
      was volume dependent. These results show that STBT inhibits CIA and may be 
      correlated with TLR4, MyD88 and NF-kappaB expression.
FAU - Xu, Wei
AU  - Xu W
AD  - Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou, 
      Fujian 350122, P.R. China.
FAU - Li, Huang
AU  - Li H
FAU - Chu, Kedan
AU  - Chu K
FAU - Zhang, Yuqin
AU  - Zhang Y
FAU - Zheng, Haiyin
AU  - Zheng H
FAU - Huang, Mingqing
AU  - Huang M
FAU - Zhang, Xun
AU  - Zhang X
FAU - Chen, Lidian
AU  - Chen L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130918
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Collagen Type II)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Myd88 protein, rat)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (NF-kappa B)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tlr4 protein, rat)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Arthritis, Experimental/chemically induced/*drug therapy/metabolism
MH  - Blotting, Western
MH  - Cattle
MH  - Cell Proliferation/drug effects
MH  - Collagen Type II/*toxicity
MH  - *Disease Models, Animal
MH  - Drugs, Chinese Herbal/*pharmacology
MH  - Inflammation Mediators/metabolism
MH  - Male
MH  - *Medicine, Chinese Traditional
MH  - Myeloid Differentiation Factor 88/genetics/metabolism
MH  - NF-kappa B/genetics/metabolism
MH  - *Phytotherapy
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Real-Time Polymerase Chain Reaction
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Synovial Membrane/drug effects/metabolism
MH  - Toll-Like Receptor 4/genetics/metabolism
EDAT- 2013/09/26 06:00
MHDA- 2014/11/14 06:00
CRDT- 2013/09/26 06:00
PHST- 2013/03/12 00:00 [received]
PHST- 2013/09/09 00:00 [accepted]
PHST- 2013/09/26 06:00 [entrez]
PHST- 2013/09/26 06:00 [pubmed]
PHST- 2014/11/14 06:00 [medline]
AID - 10.3892/mmr.2013.1693 [doi]
PST - ppublish
SO  - Mol Med Rep. 2013 Nov;8(5):1479-85. doi: 10.3892/mmr.2013.1693. Epub 2013 Sep 18.