PMID- 24065219
OWN - NLM
STAT- MEDLINE
DCOM- 20131125
LR  - 20130925
IS  - 1128-3602 (Print)
IS  - 1128-3602 (Linking)
VI  - 17
IP  - 17
DP  - 2013 Sep
TI  - Evaluation of the effects of hyperbaric oxygen treatment and enoxaparin on left 
      colon anastomosis. An experimental study.
PG  - 2286-92
LID - 5083 [pii]
AB  - BACKGROUND: Surgical interventions on left colon lead to high morbidity. The 
      problems in wound healing are the main cause of this morbidity. Hypoxia retards 
      wound healing and hyperbaric oxygen treatment (HBOT) has an anti-hypoxic effect. 
      MATERIALS AND METHODS: In this experimental study we divided eighty Wistar albino 
      rats into eight groups and numbered between 1 and 8. Normal (non-ischemic) and 
      ischemic left colon anastomosis were performed in the first and second four 
      groups respectively. HBOT and subcutaneous enoxaparin were applied to the groups 
      separately and in combination for four days, except the control groups. (Group-1 
      and Group-5). We measured anastomotic bursting pressures and performed 
      pathological examinations besides electron microscopic study in one sample from 
      each group after sacrificing the rats on the fourth day. RESULTS: There were no 
      statistically significant differences in bursting pressures when we compared 
      Group-1 with other non-ischemic groups, and Group-5 with Group-6, but there were 
      statistically significant differences when we compared Group-5 with Group-7 and 
      8. In pathological examination, there were no statistically significant 
      differences between the groups concerning necrosis, epithelization, granulation 
      tissue formation and collagen deposition. Statistically significant differences 
      were found in the scores of neovascularization when we compared Group-1 with 
      Group-3 and 4, and Group-5 with Group-8. Electron microscopic evaluation revealed 
      a prominent increase both in neovascularization and collagen fibers in the 
      samples taken from the groups received enoxaparine and hyperbaric oxygen 
      treatment in combination. CONCLUSIONS: These findings suggest that HBOT increases 
      neovascularization and bursting pressures in ischemic colon anastomosis in 
      contrast with enoxaparin.
FAU - Kemik, O
AU  - Kemik O
AD  - Acibadem University, International Hospital Organ Transplant Center, Istanbul, 
      Turkey. ozgurkemik@hotmail.com.
FAU - Adas, G
AU  - Adas G
FAU - Arikan, S
AU  - Arikan S
FAU - Gurluler, E
AU  - Gurluler E
FAU - Dogan, Y
AU  - Dogan Y
FAU - Toklu, A S
AU  - Toklu AS
FAU - Kapran, Y
AU  - Kapran Y
FAU - Kuntsal, L
AU  - Kuntsal L
FAU - Purisa, S
AU  - Purisa S
FAU - Kemik, A
AU  - Kemik A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Anticoagulants)
RN  - 0 (Enoxaparin)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - *Anastomosis, Surgical
MH  - Animals
MH  - Anticoagulants/pharmacology/therapeutic use
MH  - Collagen/metabolism
MH  - Colon/blood supply/pathology/*surgery
MH  - Combined Modality Therapy
MH  - Disease Models, Animal
MH  - Enoxaparin/pharmacology/*therapeutic use
MH  - Female
MH  - Hyperbaric Oxygenation/*methods
MH  - Ischemia/pathology
MH  - Microscopy, Electron
MH  - Neovascularization, Physiologic/drug effects
MH  - Rats
MH  - Rats, Wistar
EDAT- 2013/09/26 06:00
MHDA- 2013/12/16 06:00
CRDT- 2013/09/26 06:00
PHST- 2013/09/26 06:00 [entrez]
PHST- 2013/09/26 06:00 [pubmed]
PHST- 2013/12/16 06:00 [medline]
AID - 5083 [pii]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2013 Sep;17(17):2286-92.