PMID- 24066982
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20131115
LR  - 20211021
IS  - 1678-9199 (Print)
IS  - 1678-9199 (Electronic)
IS  - 1678-9180 (Linking)
VI  - 19
IP  - 1
DP  - 2013 Sep 25
TI  - Vasoconstrictor effect of Africanized honeybee (Apis mellifera L.) venom on rat 
      aorta.
PG  - 24
LID - 10.1186/1678-9199-19-24 [doi]
AB  - BACKGROUND: Apis mellifera stings are a problem for public health worldwide, 
      particularly in Latin America due to the aggressiveness of its Africanized 
      honeybees. Massive poisoning by A. mellifera venom (AmV) affects mainly the 
      cardiovascular system, and several works have described its actions on heart 
      muscle. Nevertheless, no work on the pharmacological action mechanisms of the AmV 
      in isolated aorta has been reported. Thus, the present work aimed to investigate 
      the actions of AmV and its main fractions, phospholipase A2 (PLA2) and melittin, 
      on isolated aorta rings and a probable action mechanism. RESULTS: AmV and the 
      complex PLA2 + melittin (0.1-50 mug/mL) caused contraction in 
      endothelium-containing aorta rings, but neither isolated PLA2 nor melittin were 
      able to reproduce the effect. Endothelium removal did not change the maximum 
      vasoconstrictor effect elicited by AmV. Ca2+-free medium, as well as treatment 
      with phentolamine (5 muM), verapamil (10 muM), losartan (100 muM), and U-73122 (10 
      muM, a phospholipase C inhibitor), eliminated the AmV-induced contractile effects. 
      CONCLUSIONS: In conclusion, AmV caused contractile effect in aorta rings probably 
      through the involvement of voltage-operated calcium channels, AT1 and 
      alpha-adrenergic receptors via the downstream activation of phospholipase C. The 
      protein complex, PLA2 + melittin, was also able to induce vasoconstriction, 
      whereas the isolated proteins were not.
FAU - Sousa, Paulo Cesar P
AU  - Sousa PC
AD  - Paulista Coast Experimental Campus, Sao Paulo State University (UNESP - Univ 
      Estadual Paulista), Sao Vicente, Sao Paulo State, Brazil. mhtjpn@yahoo.com.
FAU - Brito, Teresinha S
AU  - Brito TS
FAU - Freire, Daniel S
AU  - Freire DS
FAU - Ximenes, Rafael M
AU  - Ximenes RM
FAU - Magalhaes, Pedro Jorge C
AU  - Magalhaes PJ
FAU - Monteiro, Helena Sa
AU  - Monteiro HS
FAU - Alves, Renata S
AU  - Alves RS
FAU - Martins, Alice Maria C
AU  - Martins AM
FAU - Toyama, Daniela O
AU  - Toyama DO
FAU - Toyama, Marcos H
AU  - Toyama MH
LA  - eng
PT  - Journal Article
DEP - 20130925
PL  - Brazil
TA  - J Venom Anim Toxins Incl Trop Dis
JT  - The journal of venomous animals and toxins including tropical diseases
JID - 101201501
PMC - PMC3849866
EDAT- 2013/09/27 06:00
MHDA- 2013/09/27 06:01
PMCR- 2013/09/25
CRDT- 2013/09/27 06:00
PHST- 2013/04/06 00:00 [received]
PHST- 2013/09/03 00:00 [accepted]
PHST- 2013/09/27 06:00 [entrez]
PHST- 2013/09/27 06:00 [pubmed]
PHST- 2013/09/27 06:01 [medline]
PHST- 2013/09/25 00:00 [pmc-release]
AID - 1678-9199-19-24 [pii]
AID - 10.1186/1678-9199-19-24 [doi]
PST - epublish
SO  - J Venom Anim Toxins Incl Trop Dis. 2013 Sep 25;19(1):24. doi: 
      10.1186/1678-9199-19-24.