PMID- 24067468
OWN - NLM
STAT- MEDLINE
DCOM- 20140328
LR  - 20170214
IS  - 0394-6320 (Print)
IS  - 0394-6320 (Linking)
VI  - 26
IP  - 3
DP  - 2013 Jul-Sep
TI  - HIV impairs CD34+-derived monocytic precursor differentiation into functional 
      dendritic cells.
PG  - 717-24
AB  - Dendritic cells (DCs) perform a basic role in the immune system by allowing the 
      initiation of the primary T-cell-dependent immune response. Given previous 
      indirect evidence that DC maturation and function are impaired by HIV, we have 
      developed an in vitro culture system in order to verify the effect of HIV 
      infection on DC function during the development from hematopoietic progenitors. 
      Considering that monocytic (Mo) differentiating cells efficiently replicate 
      monocytotropic HIV, we examined whether HIV-infected monocytic precursors (MoP) 
      were able to generate functional DCs. CD34+ hematopoietic progenitor cells (HPCs) 
      were induced along Mo differentiative pathway in liquid cultures and at an early 
      stage of culture, MoP were infected with M-tropic BaL HIV strain, and after 2 
      days they were switched to DC differentiation with GM-CSF and IL-4. Derived DCs 
      were actively infected, as detected by HIV-p24 production. HIV did not 
      significantly affect cell viability, but induced a reduction in cell 
      proliferation and an inefficient functional activity in terms of uptake 
      capability and stimulation of allogenic T cells. These results indicate that 
      HIV-infected MoP lost the capacity to generate functional DCs, and this may 
      represent one of the many mechanisms of immunosuppression exploited by HIV.
FAU - Bordoni, V
AU  - Bordoni V
AD  - Cellular Immunology Laboratory, National Institute for Infectious Diseases 
      L.Spallanzani, IRCCS, Rome, Italy.
FAU - Castelli, G
AU  - Castelli G
FAU - Montesoro, E
AU  - Montesoro E
FAU - Federico, M
AU  - Federico M
FAU - Sacchi, A
AU  - Sacchi A
FAU - Morsilli, O
AU  - Morsilli O
FAU - Agrati, C
AU  - Agrati C
FAU - Martini, F
AU  - Martini F
FAU - Chelucci, C
AU  - Chelucci C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Int J Immunopathol Pharmacol
JT  - International journal of immunopathology and pharmacology
JID - 8911335
RN  - 0 (Antigens, CD34)
RN  - 0 (Biomarkers)
RN  - 0 (HIV Core Protein p24)
RN  - 0 (p24 protein, Human Immunodeficiency Virus Type 1)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Antigens, CD34/*metabolism
MH  - Biomarkers/metabolism
MH  - *Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Dendritic Cells/immunology/metabolism/*virology
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/metabolism
MH  - HIV Core Protein p24/metabolism
MH  - HIV-1/immunology/metabolism/*pathogenicity
MH  - Hematopoietic Stem Cells/immunology/metabolism/*virology
MH  - Humans
MH  - Immunocompromised Host
MH  - Interleukin-4/metabolism
MH  - T-Lymphocytes/immunology
MH  - Time Factors
EDAT- 2013/09/27 06:00
MHDA- 2014/03/29 06:00
CRDT- 2013/09/27 06:00
PHST- 2013/09/27 06:00 [entrez]
PHST- 2013/09/27 06:00 [pubmed]
PHST- 2014/03/29 06:00 [medline]
AID - 15 [pii]
AID - 10.1177/039463201302600315 [doi]
PST - ppublish
SO  - Int J Immunopathol Pharmacol. 2013 Jul-Sep;26(3):717-24. doi: 
      10.1177/039463201302600315.