PMID- 24067896
OWN - NLM
STAT- MEDLINE
DCOM- 20150330
LR  - 20220129
IS  - 1477-0970 (Electronic)
IS  - 1352-4585 (Linking)
VI  - 20
IP  - 6
DP  - 2014 May
TI  - Reductions in neuronal peroxisomes in multiple sclerosis grey matter.
PG  - 651-9
LID - 10.1177/1352458513505691 [doi]
AB  - BACKGROUND: Peroxisomes are organelles in eukaryotic cells with multiple 
      functions including the detoxification of reactive oxygen species, plasmalogen 
      synthesis and beta-oxidation of fatty acids. Recent evidence has implicated 
      peroxisomal dysfunction in models of multiple sclerosis (MS) disease progression. 
      OBJECTIVES: Our aims were to determine whether there are changes in peroxisomes 
      in MS grey matter (GM) compared to control GM. METHODS: We analysed cases of MS 
      and control GM immunocytochemically to assess peroxisomal membrane protein 
      (PMP70) and neuronal proteins. We examined the expression of ABCD3 (the gene that 
      encodes PMP70) in MS and control GM. Analyses of very long chain fatty acid 
      (VLCFA) levels in GM were performed. RESULTS: PMP70 immunolabelling of neuronal 
      somata was significantly lower in MS GM compared to control. Calibration of ABCD3 
      gene expression with reference to glyceraldehyde 3-phsophate dehydrogenase 
      (GAPDH) revealed overall decreases in expression in MS compared to controls. Mean 
      PMP70 counts in involved MS GM negatively correlated to disease duration. 
      Elevations in C26:0 (hexacosanoic acid) were found in MS GM. CONCLUSIONS: 
      Collectively, these observations provide evidence that there is an overall 
      reduction in peroxisomal gene expression and peroxisomal proteins in GM neurons 
      in MS. Changes in peroxisomal function may contribute to neuronal dysfunction and 
      degeneration in MS.
FAU - Gray, Elizabeth
AU  - Gray E
AD  - MS and Stem Cell Labs, University of Bristol, UK.
FAU - Rice, Claire
AU  - Rice C
FAU - Hares, Kelly
AU  - Hares K
FAU - Redondo, Juliana
AU  - Redondo J
FAU - Kemp, Kevin
AU  - Kemp K
FAU - Williams, Marcus
AU  - Williams M
FAU - Brown, Ann
AU  - Brown A
FAU - Scolding, Neil
AU  - Scolding N
FAU - Wilkins, Alastair
AU  - Wilkins A
LA  - eng
GR  - G-0909/PUK_/Parkinson's UK/United Kingdom
GR  - MR/K004166/1/MRC_/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130925
PL  - England
TA  - Mult Scler
JT  - Multiple sclerosis (Houndmills, Basingstoke, England)
JID - 9509185
RN  - 0 (ABCD3 protein, human)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Membrane Proteins)
SB  - IM
MH  - ATP-Binding Cassette Transporters/metabolism
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Female
MH  - Gene Expression/physiology
MH  - Gray Matter/metabolism/*pathology
MH  - Humans
MH  - Male
MH  - Membrane Proteins/*metabolism
MH  - Middle Aged
MH  - Multiple Sclerosis/*pathology
MH  - Neurons/metabolism/*pathology
MH  - Peroxisomes/*pathology
OTO - NOTNLM
OT  - Peroxisomes
OT  - grey matter
OT  - multiple sclerosis
EDAT- 2013/09/27 06:00
MHDA- 2015/03/31 06:00
CRDT- 2013/09/27 06:00
PHST- 2013/09/27 06:00 [entrez]
PHST- 2013/09/27 06:00 [pubmed]
PHST- 2015/03/31 06:00 [medline]
AID - 1352458513505691 [pii]
AID - 10.1177/1352458513505691 [doi]
PST - ppublish
SO  - Mult Scler. 2014 May;20(6):651-9. doi: 10.1177/1352458513505691. Epub 2013 Sep 
      25.