PMID- 24068884
OWN - NLM
STAT- MEDLINE
DCOM- 20140415
LR  - 20220321
IS  - 1537-744X (Electronic)
IS  - 2356-6140 (Print)
IS  - 1537-744X (Linking)
VI  - 2013
DP  - 2013
TI  - Hypoxic culture conditions as a solution for mesenchymal stem cell based 
      regenerative therapy.
PG  - 632972
LID - 10.1155/2013/632972 [doi]
LID - 632972
AB  - Cell-based regenerative therapies, based on in vitro propagation of stem cells, 
      offer tremendous hope to many individuals suffering from degenerative diseases 
      that were previously deemed untreatable. Due to the self-renewal capacity, 
      multilineage potential, and immunosuppressive property, mesenchymal stem cells 
      (MSCs) are considered as an attractive source of stem cells for regenerative 
      therapies. However, poor growth kinetics, early senescence, and genetic 
      instability during in vitro expansion and poor engraftment after transplantation 
      are considered to be among the major disadvantages of MSC-based regenerative 
      therapies. A number of complex inter- and intracellular interactive signaling 
      systems control growth, multiplication, and differentiation of MSCs in their 
      niche. Common laboratory conditions for stem cell culture involve ambient O(2) 
      concentration (20%) in contrast to their niche where they usually reside in 2-9% 
      O(2). Notably, O(2) plays an important role in maintaining stem cell fate in terms of 
      proliferation and differentiation, by regulating hypoxia-inducible factor-1 
      (HIF-1) mediated expression of different genes. This paper aims to describe and 
      compare the role of normoxia (20% O(2)) and hypoxia (2-9% O(2)) on the biology of 
      MSCs. Finally it is concluded that a hypoxic environment can greatly improve 
      growth kinetics, genetic stability, and expression of chemokine receptors during 
      in vitro expansion and eventually can increase efficiency of MSC-based 
      regenerative therapies.
FAU - Haque, Nazmul
AU  - Haque N
AD  - Regenerative Dentistry Research Group, Faculty of Dentistry, University of 
      Malaya, 50603 Kuala Lumpur, Malaysia ; Department of Conservative Dentistry, 
      Faculty of Dentistry, University of Malaya, 50603 Kuala Lumpur, Malaysia.
FAU - Rahman, Mohammad Tariqur
AU  - Rahman MT
FAU - Abu Kasim, Noor Hayaty
AU  - Abu Kasim NH
FAU - Alabsi, Aied Mohammed
AU  - Alabsi AM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
DEP - 20130827
PL  - United States
TA  - ScientificWorldJournal
JT  - TheScientificWorldJournal
JID - 101131163
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Receptors, Chemokine)
RN  - 0 (Receptors, Notch)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Cell Culture Techniques
MH  - Cell Hypoxia
MH  - Cell Proliferation
MH  - Genomic Instability
MH  - Mesenchymal Stem Cells/*cytology
MH  - Models, Biological
MH  - Oxygen/metabolism
MH  - Reactive Oxygen Species/metabolism
MH  - Receptors, Chemokine/genetics/metabolism
MH  - Receptors, Notch/metabolism
MH  - Regenerative Medicine/methods
MH  - Signal Transduction
MH  - Up-Regulation
PMC - PMC3771429
EDAT- 2013/09/27 06:00
MHDA- 2014/04/16 06:00
PMCR- 2013/08/27
CRDT- 2013/09/27 06:00
PHST- 2013/07/03 00:00 [received]
PHST- 2013/07/26 00:00 [accepted]
PHST- 2013/09/27 06:00 [entrez]
PHST- 2013/09/27 06:00 [pubmed]
PHST- 2014/04/16 06:00 [medline]
PHST- 2013/08/27 00:00 [pmc-release]
AID - 10.1155/2013/632972 [doi]
PST - epublish
SO  - ScientificWorldJournal. 2013 Aug 27;2013:632972. doi: 10.1155/2013/632972. 
      eCollection 2013.