PMID- 24071688
OWN - NLM
STAT- MEDLINE
DCOM- 20150717
LR  - 20220321
IS  - 1672-7347 (Print)
IS  - 1672-7347 (Linking)
VI  - 38
IP  - 9
DP  - 2013 Sep
TI  - [Effect of metformin on the expression of SIRT1 and UCP2 in rat liver of type 2 
      diabetes mellitus and nonalcoholic fatty liver].
PG  - 882-7
LID - 10.3969/j.issn.1672-7347.2013.09.003 [doi]
AB  - OBJECTIVE: To observe the effect of metformin on the expression of SIRT1 and UCP2 
      in rat liver of type 2 diabetes mellitus (T2DM) with nonalcoholic fatty liver 
      disease (NAFLD), and discuss the pathogenesis of T2DM with NAFLD, and the 
      treatment with and possible mechanism of metformin. METHODS: Thirty-six male SD 
      rats were randomly divided into a normal control group (group NC, n=12), a T2DM 
      with NAFLD group (group MC, n=12), and a metformin group (group A, n=12). We 
      established the model of T2DM with NAFLD rats by feeding high-fat and high-sugar 
      diet and injecting STZ. After the success establishment of the model, the 
      metformin group was given metformin 300 mg/(kg.d) for 8 weeks. At the end of the 
      experiment, we measured FBG, ALT, AST, TC, TG, HDL-C, LDL-C, VLDL, FFAs, FINs and 
      HOMA-IR respectively in group NC, MC and A. We observed the change of liver 
      tissue pathology by HE, determined the expression of SIRT1 and UCP2 in rat liver 
      by immunohistochemical method and real-time quantitative method. RESULTS: FBG, 
      ALT, AST, TC, TG, LDL-C, VLDL, FFAs, FINs and HOMA-IR were higher in group MC 
      than in group NC (P<0.05), while HDL-C was obviously lower in group MC than in 
      group NC (P<0.05). After the metformin treatment, the serum parameters in the 
      rats had improved in group NC compared with in group MC (P<0.05). On 
      immunohistochemical staining and mRNA level, the expression of SIRT1 was 
      obviously lower in group MC than in group NC (P<0.05), and the expression of UCP2 
      was obviously higher in group MC than in group NC (P<0.05). After the metformin 
      treatment, the expression of SIRT1 was higher than in group MC (P<0.05), and the 
      expression of UCP2 was lower than in group MC (P<0.05). There was negative 
      correlation between the expression of SIRT1 and UCP2 (r=-0.61, P<0.01). 
      CONCLUSION: The expression of SIRT1 is low and the expression of UCP2 is high in 
      rat liver of T2DM with NAFLD. Metformin can increase the expression of SIRT1 and 
      reduce the expression of UCP2, with negative correlation between the expression 
      of SIRT1 and UCP2.
FAU - Xu, Jing
AU  - Xu J
AD  - Department of Endocrinology, Second Affiliated Hospital, Medical School of Xi'an 
      Jiaotong University, Xi'an 710004,China xujingjdey85@163.com.
FAU - Li, Nan
AU  - Li N
FAU - Wang, Junhong
AU  - Wang J
FAU - Zhang, Chunhong
AU  - Zhang C
FAU - Ding, Shimei
AU  - Ding S
FAU - Jiao, Yang
AU  - Jiao Y
FAU - Zhang, Jing
AU  - Zhang J
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhong Nan Da Xue Xue Bao Yi Xue Ban
JT  - Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. 
      Medical sciences
JID - 101230586
RN  - 0 (Ion Channels)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Ucp2 protein, rat)
RN  - 0 (Uncoupling Protein 2)
RN  - 9100L32L2N (Metformin)
RN  - EC 3.5.1.- (Sirt1 protein, rat)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - Animals
MH  - Diabetes Mellitus, Type 2/drug therapy/*metabolism
MH  - Ion Channels/*metabolism
MH  - Male
MH  - Metformin/*pharmacology
MH  - Mitochondrial Proteins/*metabolism
MH  - Non-alcoholic Fatty Liver Disease/drug therapy/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Sirtuin 1/*metabolism
MH  - Uncoupling Protein 2
EDAT- 2013/09/28 06:00
MHDA- 2015/07/18 06:00
CRDT- 2013/09/28 06:00
PHST- 2013/09/28 06:00 [entrez]
PHST- 2013/09/28 06:00 [pubmed]
PHST- 2015/07/18 06:00 [medline]
AID - 10.3969/j.issn.1672-7347.2013.09.003 [doi]
PST - ppublish
SO  - Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2013 Sep;38(9):882-7. doi: 
      10.3969/j.issn.1672-7347.2013.09.003.