PMID- 24072482
OWN - NLM
STAT- MEDLINE
DCOM- 20141006
LR  - 20211021
IS  - 1559-1166 (Electronic)
IS  - 0895-8696 (Linking)
VI  - 52
IP  - 2
DP  - 2014 Feb
TI  - Activating transcription factor 4 modulates BDNF release from microglial cells.
PG  - 225-30
LID - 10.1007/s12031-013-0126-1 [doi]
AB  - Pathogenic pain is a common sign of many diseases. The mechanism is unclear. 
      Activating transcription factor 4 (ATF4) plays a critical role in cell 
      activation. Brain-derived neurotrophic factor (BDNF) is an important molecule in 
      pathogenic pain. This study aims to investigate the role of ATF4 in inducing BDNF 
      release from microglial cells. In this study, mouse microglial cells were 
      cultured. The levels of BDNF in the culture medium were determined by 
      enzyme-linked immunosorbent assay. Overexpression of ATF4 in microglial cells was 
      performed by gene transfection. The apoptosis of microglial cells was assessed by 
      flow cytometry. The results showed that microglial cells expressed ATF4 and 
      protease-activated receptor-2 (PAR2). BDNF was detectable in the culture medium 
      of microglial cells, which was significantly increased in the ATF4-overexpressing 
      microglial cells. The ATF4-overexpressing microglial cells showed a high 
      frequency of apoptotic cells, which could be inhibited by exposure to the PAR2 
      agonist tryptase in the culture. The tryptase-treated ATF4-overexpressing 
      microglial cells kept higher secretion of BDNF. We conclude that the activation 
      of ATF4 can increase BDNF release from microglial cells.
FAU - Sun, Pengling
AU  - Sun P
AD  - Department of Anesthesiology, Neuroscience Research Centre, Changzheng Hospital, 
      Second Military Medical University, Shanghai, 200003, China.
FAU - Li, Xiangnan
AU  - Li X
FAU - Chen, Chengwen
AU  - Chen C
FAU - Chen, Qianbo
AU  - Chen Q
FAU - Ouyang, Qing
AU  - Ouyang Q
FAU - Liu, Fangting
AU  - Liu F
FAU - Xiang, Zhenghua
AU  - Xiang Z
FAU - Yuan, Hongbin
AU  - Yuan H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130927
PL  - United States
TA  - J Mol Neurosci
JT  - Journal of molecular neuroscience : MN
JID - 9002991
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Receptor, PAR-2)
RN  - 145891-90-3 (Activating Transcription Factor 4)
SB  - IM
MH  - Activating Transcription Factor 4/genetics/*metabolism
MH  - Animals
MH  - Apoptosis
MH  - Brain-Derived Neurotrophic Factor/genetics/*metabolism
MH  - Cell Line
MH  - Mice
MH  - Microglia/*metabolism/physiology
MH  - Receptor, PAR-2/genetics/metabolism
EDAT- 2013/09/28 06:00
MHDA- 2014/10/07 06:00
CRDT- 2013/09/28 06:00
PHST- 2013/08/03 00:00 [received]
PHST- 2013/09/16 00:00 [accepted]
PHST- 2013/09/28 06:00 [entrez]
PHST- 2013/09/28 06:00 [pubmed]
PHST- 2014/10/07 06:00 [medline]
AID - 10.1007/s12031-013-0126-1 [doi]
PST - ppublish
SO  - J Mol Neurosci. 2014 Feb;52(2):225-30. doi: 10.1007/s12031-013-0126-1. Epub 2013 
      Sep 27.