PMID- 24075408
OWN - NLM
STAT- MEDLINE
DCOM- 20140521
LR  - 20211021
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 34
IP  - 38
DP  - 2013 Dec
TI  - Mutant MCP-1 protein delivery from layer-by-layer coatings on orthopedic implants 
      to modulate inflammatory response.
PG  - 10287-95
LID - S0142-9612(13)01111-3 [pii]
LID - 10.1016/j.biomaterials.2013.09.028 [doi]
AB  - Total joint replacement (TJR) is a common and effective surgical procedure for 
      hip or knee joint reconstruction. However, the production of wear particles is 
      inevitable for all TJRs, which activates macrophages and initiates an 
      inflammatory cascade often resulting in bone loss, prosthetic loosening and 
      eventual TJR failure. Macrophage Chemoattractant Protein-1 (MCP-1) is one of the 
      most potent cytokines responsible for macrophage cell recruitment, and previous 
      studies suggest that mutant MCP-1 proteins such as 7ND may be used as a decoy 
      drug to block the receptor and reduce inflammatory cell recruitment. Here we 
      report the development of a biodegradable, layer-by-layer (LBL) coating platform 
      that allows efficient loading and controlled release of 7ND proteins from the 
      surface of orthopedic implants using as few as 14 layers. Scanning electron 
      microscopy and fluorescence imaging confirmed effective coating using the LBL 
      procedure on titanium rods. 7ND protein loading concentration and release 
      kinetics can be modulated by varying the polyelectrolytes of choice, the polymer 
      chemistry, the pH of the polyelectrolyte solution, and the degradation rate of 
      the LBL assembly. The released 7ND from LBL coating retained its bioactivity and 
      effectively reduced macrophage migration towards MCP-1. Finally, the LBL coating 
      remained intact following a femoral rod implantation procedure as determined by 
      immunostaining of the 7ND coating. The LBL platform reported herein may be 
      applied for in situ controlled release of 7ND protein from orthopedic implants, 
      to reduce wear particle-induced inflammatory responses in an effort to prolong 
      the lifetime of implants.
CI  - Copyright (c) 2013 Elsevier Ltd. All rights reserved.
FAU - Keeney, Michael
AU  - Keeney M
AD  - Department of Orthopaedic Surgery, Stanford University, Stanford, CA 94305, USA. 
      Electronic address: mkeeney@stanford.edu.
FAU - Waters, Heather
AU  - Waters H
FAU - Barcay, Katherine
AU  - Barcay K
FAU - Jiang, Xinyi
AU  - Jiang X
FAU - Yao, Zhenyu
AU  - Yao Z
FAU - Pajarinen, Jukka
AU  - Pajarinen J
FAU - Egashira, Kensuke
AU  - Egashira K
FAU - Goodman, Stuart B
AU  - Goodman SB
FAU - Yang, Fan
AU  - Yang F
LA  - eng
GR  - R01 AR055650/AR/NIAMS NIH HHS/United States
GR  - R01 AR063717/AR/NIAMS NIH HHS/United States
GR  - 2R01AR055650-05/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130926
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Chemokine CCL2)
SB  - IM
MH  - Animals
MH  - Cell Line
MH  - Chemokine CCL2/*administration & dosage/*chemistry
MH  - Female
MH  - Humans
MH  - Inflammation/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Orthopedics/*methods
MH  - *Prostheses and Implants
PMC - PMC4018195
MID - NIHMS524965
OTO - NOTNLM
OT  - Controlled drug release
OT  - Inflammation
OT  - Macrophage
OT  - Titanium
OT  - Wear debris
EDAT- 2013/10/01 06:00
MHDA- 2014/05/23 06:00
PMCR- 2014/12/01
CRDT- 2013/10/01 06:00
PHST- 2013/08/19 00:00 [received]
PHST- 2013/09/09 00:00 [accepted]
PHST- 2013/10/01 06:00 [entrez]
PHST- 2013/10/01 06:00 [pubmed]
PHST- 2014/05/23 06:00 [medline]
PHST- 2014/12/01 00:00 [pmc-release]
AID - S0142-9612(13)01111-3 [pii]
AID - 10.1016/j.biomaterials.2013.09.028 [doi]
PST - ppublish
SO  - Biomaterials. 2013 Dec;34(38):10287-95. doi: 10.1016/j.biomaterials.2013.09.028. 
      Epub 2013 Sep 26.