PMID- 24077781
OWN - NLM
STAT- MEDLINE
DCOM- 20150723
LR  - 20211021
IS  - 1435-5922 (Electronic)
IS  - 0944-1174 (Linking)
VI  - 49
IP  - 10
DP  - 2014 Oct
TI  - Immunoregulatory function of PIR-A/B+ DCs in the inflammatory responses of 
      dextran sodium sulfate-induced colitis.
PG  - 1367-77
LID - 10.1007/s00535-013-0879-x [doi]
AB  - BACKGROUND: Dendritic cells (DCs) may play an important role in forms of 
      inflammatory bowel disease (IBD), such as Crohn's disease and ulcerative colitis. 
      DCs are generally recognized as initiators of acquired immunity and also serve as 
      regulators of both innate and acquired immunity. We used the animal model of 
      colitis induced by dextran sodium sulfate (DSS), and examined whether DCs 
      prepared from the colon show immunoregulatory roles in the termination of 
      DSS-induced colitis. METHODS: C57BL/6 mice exposed to DSS for 5 days developed 
      acute colitis. DCs were isolated from the large intestinal lamina propria, and 
      then analyzed for phenotypical, functional, and genetic data. RESULTS: Only 
      PIR-A/B(low) conventional DCs (cDCs) were detected in the steady state. However, 
      after the treatment of DSS, PIR-A/B(high) cDCs appeared and gradually increased 
      from day 5 to day 7, at which time the DSS-induced colitis was terminated. Then, 
      allogeneic mixed leukocyte reaction (MLR) was performed. The stimulatory activity 
      of PIR-A/B(high) cDCs obtained on day 7 was very low, and the addition of 
      PIR-A/B(high) cDCs suppressed the T cell proliferation in MLR, indicating the 
      immunoregulatory role of PIR-A/B(high) cDCs. The immunoregulatory role of 
      PIR-A/B(high) cDCs was confirmed by the in vivo transfer experiment, showing 
      their therapeutic effect on DSS-induced colitis. The message level of TGFbetai was 
      significantly higher in PIR-A/B(high) cDCs, while that of IFN-gamma was highly 
      upregulated in PIR-A/B(low) cDCs, being well in accordance with the fact that 
      PIR-A/B(high) cDCs showed a suppressive function against activated T cells. 
      CONCLUSION: PIR-A/B(high) cDCs showed a suppressive function against activated T 
      cells by producing inhibitory cytokines.
FAU - Kurishima, Akiko
AU  - Kurishima A
AD  - Division of Gastroenterology and Hepatology, Third Department of Internal 
      Medicine, Kansai Medical University, 10-15 Fumizono-cho, Moriguchi, Osaka, 
      570-8506, Japan, kurishia@takii.kmu.ac.jp.
FAU - Inaba, Muneo
AU  - Inaba M
FAU - Sakaguchi, Yutaku
AU  - Sakaguchi Y
FAU - Fukui, Toshiro
AU  - Fukui T
FAU - Uchida, Kazushige
AU  - Uchida K
FAU - Nishio, Akiyoshi
AU  - Nishio A
FAU - Nomura, Shosaku
AU  - Nomura S
FAU - Okazaki, Kazuichi
AU  - Okazaki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20130929
PL  - Japan
TA  - J Gastroenterol
JT  - Journal of gastroenterology
JID - 9430794
RN  - 0 (Receptors, Immunologic)
RN  - 9042-14-2 (Dextran Sulfate)
SB  - IM
MH  - Adoptive Transfer
MH  - Animals
MH  - Colon/immunology
MH  - Dendritic Cells/*immunology/transplantation
MH  - Dextran Sulfate
MH  - Disease Models, Animal
MH  - Female
MH  - Inflammatory Bowel Diseases/chemically induced/*immunology/pathology/therapy
MH  - Lymphocyte Culture Test, Mixed/methods
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Receptors, Immunologic/analysis
EDAT- 2013/10/01 06:00
MHDA- 2015/07/24 06:00
CRDT- 2013/10/01 06:00
PHST- 2013/01/21 00:00 [received]
PHST- 2013/09/02 00:00 [accepted]
PHST- 2013/10/01 06:00 [entrez]
PHST- 2013/10/01 06:00 [pubmed]
PHST- 2015/07/24 06:00 [medline]
AID - 10.1007/s00535-013-0879-x [doi]
PST - ppublish
SO  - J Gastroenterol. 2014 Oct;49(10):1367-77. doi: 10.1007/s00535-013-0879-x. Epub 
      2013 Sep 29.