PMID- 24077878
OWN - NLM
STAT- MEDLINE
DCOM- 20140928
LR  - 20211021
IS  - 1573-6830 (Electronic)
IS  - 0272-4340 (Linking)
VI  - 34
IP  - 1
DP  - 2014 Jan
TI  - The expression changes of vacuolar protein sorting 4B (VPS4B) following middle 
      cerebral artery occlusion (MCAO) in adult rats brain hippocampus.
PG  - 83-94
AB  - Vacuolar protein sorting 4 (VPS4), is a member of ATPases associated with diverse 
      cellular activities protein family. VPS4 is composed of VPS4A and VPS4B, VPS4B 
      plays an important role in the lysosomal degradation pathway, intracellular 
      protein trafficking, virus budding and abscission of cytokinesis. However, 
      information regarding its distribution and possible function in the central 
      nervous system is limited. Therefore, we performed a middle cerebral artery 
      occlusion (MCAO) in adult rats and detected the dynamic changes of VPS4B in 
      hippocampus CA1 subregion. We found that the VPS4B expression was increased 
      strongly after MCAO and reached the peak after 3 days. VPS4B mainly located in 
      the cytoplasm of neurons, but not astrocytes and microglia. Moreover, there was a 
      concomitant up-regulation of active caspase-3. In vitro studies indicated that 
      the up-regulation of VPS4B may be involved in oxygen-glucose deprivation-induced 
      PC12 cell death. And knock-down of VPS4B in cultured differentiated PC12 cells by 
      siRNA showed that VPS4B promoted the expression of active caspase-3. 
      Collectively, all these results and MTT assay suggested that the up-regulation of 
      VPS4B played an important role in the pathophysiology after MCAO, and further 
      research is needed to have a good understanding of its function and mechanism.
FAU - Cui, Gang
AU  - Cui G
FAU - Wang, Yunfeng
AU  - Wang Y
FAU - Yu, Shanshan
AU  - Yu S
FAU - Yang, Lixiang
AU  - Yang L
FAU - Li, Bing
AU  - Li B
FAU - Wang, Wei
AU  - Wang W
FAU - Zhou, Peng
AU  - Zhou P
FAU - Wu, Jiang
AU  - Wu J
FAU - Lu, Ting
AU  - Lu T
FAU - Chen, Dongjian
AU  - Chen D
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cell Mol Neurobiol
JT  - Cellular and molecular neurobiology
JID - 8200709
RN  - 0 (Biomarkers)
RN  - 0 (Vesicular Transport Proteins)
RN  - EC 3.4.22.- (Caspase 3)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Apoptosis
MH  - Biomarkers/metabolism
MH  - Blotting, Western
MH  - CA1 Region, Hippocampal/enzymology/pathology
MH  - Caspase 3/metabolism
MH  - Cell Survival
MH  - Gene Knockdown Techniques
MH  - Glucose/deficiency
MH  - Hippocampus/*metabolism/*pathology
MH  - Hypoxia/metabolism/pathology
MH  - Immunohistochemistry
MH  - Infarction, Middle Cerebral Artery/*metabolism/pathology
MH  - Male
MH  - Neurons/metabolism/pathology
MH  - PC12 Cells
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Up-Regulation
MH  - Vesicular Transport Proteins/*metabolism
EDAT- 2013/10/01 06:00
MHDA- 2014/10/01 06:00
CRDT- 2013/10/01 06:00
PHST- 2013/07/14 00:00 [received]
PHST- 2013/09/17 00:00 [accepted]
PHST- 2013/10/01 06:00 [entrez]
PHST- 2013/10/01 06:00 [pubmed]
PHST- 2014/10/01 06:00 [medline]
AID - 10.1007/s10571-013-9989-5 [doi]
PST - ppublish
SO  - Cell Mol Neurobiol. 2014 Jan;34(1):83-94. doi: 10.1007/s10571-013-9989-5.