PMID- 24078697
OWN - NLM
STAT- MEDLINE
DCOM- 20140114
LR  - 20220120
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 191
IP  - 9
DP  - 2013 Nov 1
TI  - Characterization of human afferent lymph dendritic cells from seroma fluids.
PG  - 4858-66
LID - 10.4049/jimmunol.1300760 [doi]
AB  - Dendritic cells (DCs) migrate from peripheral tissues to secondary lymphoid 
      organs (SLOs) through the afferent lymph. Owing to limitations in investigating 
      human lymph, DCs flowing in afferent lymph have not been properly characterized 
      in humans until now. In this study, DCs present in seroma, an accrual of human 
      afferent lymph occurring after lymph node surgical dissection, were isolated and 
      analyzed in detail. Two main DC subsets were identified in seroma that 
      corresponded to the migratory DC subsets present in lymph nodes, that is, CD14(+) 
      and CD1a(+). The latter also included CD1a(bright) Langerhans cells. The two DC 
      subsets appeared to share the same monocytic precursor and to be developmentally 
      related; both of them spontaneously released high levels of TGF-beta and displayed 
      similar T cell-activating and -polarizing properties. In contrast, they differed 
      in the expression of surface molecules, including TLRs; in their phagocytic 
      activity; and in the expression of proteins involved in Ag processing and 
      presentation. It is worth noting that although both subsets were detected in 
      seroma in the postsurgical inflammatory phase, only CD1a(+) DCs migrated via 
      afferent lymph under steady-state conditions. In conclusion, the high numbers of 
      DCs contained in seroma fluids allowed a proper characterization of human DCs 
      migrating via afferent lymph, revealing a continuous stream of DCs from 
      peripheral regions toward SLOs under normal conditions. Moreover, we showed that, 
      in inflammatory conditions, distinct subsets of DCs can migrate to SLOs via 
      afferent lymph.
FAU - Morandi, Barbara
AU  - Morandi B
AD  - Department of Experimental Medicine, University of Genoa, 16132 Genoa, Italy;
FAU - Bonaccorsi, Irene
AU  - Bonaccorsi I
FAU - Mesiti, Mario
AU  - Mesiti M
FAU - Conte, Romana
AU  - Conte R
FAU - Carrega, Paolo
AU  - Carrega P
FAU - Costa, Gregorio
AU  - Costa G
FAU - Iemmo, Raffaella
AU  - Iemmo R
FAU - Martini, Stefania
AU  - Martini S
FAU - Ferrone, Soldano
AU  - Ferrone S
FAU - Cantoni, Claudia
AU  - Cantoni C
FAU - Mingari, Maria Cristina
AU  - Mingari MC
FAU - Moretta, Lorenzo
AU  - Moretta L
FAU - Ferlazzo, Guido
AU  - Ferlazzo G
LA  - eng
GR  - R01 CA110249/CA/NCI NIH HHS/United States
GR  - R01CA110249/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20130927
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1a antigen)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, CD1/*metabolism
MH  - Cell Movement/immunology
MH  - Dendritic Cells/*classification/immunology/metabolism
MH  - Female
MH  - Humans
MH  - Lipopolysaccharide Receptors/*metabolism
MH  - Lymph/*cytology
MH  - Lymph Nodes/cytology
MH  - Lymphocyte Activation/immunology
MH  - Male
MH  - Middle Aged
MH  - *Seroma
MH  - Transforming Growth Factor beta/metabolism
PMC - PMC8767499
MID - NIHMS1764482
COIS- Disclosures The authors have no financial conflicts of interest.
EDAT- 2013/10/01 06:00
MHDA- 2014/01/15 06:00
PMCR- 2022/01/19
CRDT- 2013/10/01 06:00
PHST- 2013/10/01 06:00 [entrez]
PHST- 2013/10/01 06:00 [pubmed]
PHST- 2014/01/15 06:00 [medline]
PHST- 2022/01/19 00:00 [pmc-release]
AID - jimmunol.1300760 [pii]
AID - 10.4049/jimmunol.1300760 [doi]
PST - ppublish
SO  - J Immunol. 2013 Nov 1;191(9):4858-66. doi: 10.4049/jimmunol.1300760. Epub 2013 
      Sep 27.